Document Detail


Aptamer selection express: a novel method for rapid single-step selection and sensing of aptamers.
MedLine Citation:
PMID:  19183794     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Here we describe a new DNA capture element (DCE) sensing system, based on the quenching and dequenching of a double-stranded aptamer. This system shows very good sensitivity and thermal stability. While quenching, dequenching, and separating the DCE systems made from different aptamers (all selected by SELEX), an alternative method to rapidly select aptamers was developed-the Aptamer Selection Express (ASExp). This process has been used to select aptamers against different types of targets (Bacillus anthracis spores, Bacillus thuringiensis spores, MS-2 bacteriophage, ovalbumin, and botulinum neurotoxin). The DCE systems made from botulinum neurotoxin aptamers selected by ASExp have been investigated. The results of this investigation indicate that ASExp can be used to rapidly select aptamers for the DCE sensing system.
Authors:
Maomian Fan; Shelly Roper McBurnett; Carrie J Andrews; Amity M Allman; John G Bruno; Johnathan L Kiel
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of biomolecular techniques : JBT     Volume:  19     ISSN:  1943-4731     ISO Abbreviation:  J Biomol Tech     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2009-02-02     Completed Date:  2009-04-02     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  100888641     Medline TA:  J Biomol Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  311-9     Citation Subset:  IM    
Affiliation:
Air Force Research Laboratories, RHPC, Brooks City-Base, TX, USA. maomian.fan@brooks.af.mil
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MeSH Terms
Descriptor/Qualifier:
Animals
Aptamers, Nucleotide / genetics*,  isolation & purification*
Bacillus anthracis / genetics
Base Sequence
Biotechnology
Botulinum Toxins / genetics
Levivirus / genetics
Ovalbumin / genetics
Polymerase Chain Reaction
SELEX Aptamer Technique / methods*
Spores, Bacterial / genetics
Grant Support
ID/Acronym/Agency:
1R43AI058561-01/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Aptamers, Nucleotide; 9006-59-1/Ovalbumin; EC 3.4.24.69/Botulinum Toxins
Comments/Corrections

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