Document Detail


Aptamer directly evolved from live cells recognizes membrane bound immunoglobin heavy mu chain in Burkitt's lymphoma cells.
MedLine Citation:
PMID:  17875608     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The identification of tumor related cell membrane protein targets is important in understanding tumor progression, the development of new diagnostic tools, and potentially for identifying new therapeutic targets. Here we present a novel strategy for identifying proteins that are altered in their expression levels in a diseased cell using cell specific aptamers. Using an intact viable B-cell Burkitt's lymphoma cell line (Ramos cells) as the target, we have selected aptamers that recognize cell membrane proteins with high affinity. Among the selected aptamers that showed different recognition patterns with different cell lines of leukemia, the aptamer TD05 showed binding with Ramos cells. By chemically modifying TD05 to covalently cross-link with its target on Ramos cells to capture and to enrich the target receptors using streptavidin coated magnetic beads followed by mass spectrometry, we were able to identify membrane bound immunoglobin heavy mu chain as the target for TD05 aptamer. Immunoglobin heavy mu chain is a major component of the B-cell antigen receptor, which is expressed in Burkitt's lymphoma cells. This study demonstrates that this two step strategy, the development of high quality aptamer probes and then the identification of their target proteins, can be used to discover new disease related potential markers and thus enhance tumor diagnosis and therapy. The aptamer based strategy will enable effective molecular elucidation of disease related biomarkers and other interesting molecules.
Authors:
Prabodhika Mallikaratchy; Zhiwen Tang; Sefah Kwame; Ling Meng; Dihua Shangguan; Weihong Tan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-09-17
Journal Detail:
Title:  Molecular & cellular proteomics : MCP     Volume:  6     ISSN:  1535-9476     ISO Abbreviation:  Mol. Cell Proteomics     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-11     Completed Date:  2008-05-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101125647     Medline TA:  Mol Cell Proteomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2230-8     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Shands Cancer Center, University of Florida Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611, USA.
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MeSH Terms
Descriptor/Qualifier:
Aptamers, Peptide*
Base Sequence
Burkitt Lymphoma / metabolism*,  pathology
DNA Primers
Humans
Immunoglobulin mu-Chains / chemistry*
Protein Binding
Chemical
Reg. No./Substance:
0/Aptamers, Peptide; 0/DNA Primers; 0/Immunoglobulin mu-Chains

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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