Document Detail

Aptamer carbon nanodot sandwich used for fluorescent detection of protein.
MedLine Citation:
PMID:  23050264     Owner:  NLM     Status:  Publisher    
Carbon nanodots (C-Dots) have attracted growing interest in recent years due to their low cost, ready scalability, excellent chemical stability, biocompatibility, colloidal stability, and resilience of photoluminescence. They have been employed as novel, ideal fluorescent probes for bio-imaging and smart sensing. In addition, taking advantage of their low-cytotoxicity, C-Dots have potential applications in biochemical and cell biological fields. Herein, we present the first assay with aptamer-functionalized C-Dots as a sensory platform for protein detection. The presence of thrombin can induce the aptamer-modified fluorescent C-Dots to form a sandwich structure with aptamer-functionalized silica nanoparticles through specific protein/aptamer interaction. The assay shows high specificity toward thrombin. A detection limit of 1 nM is obtained, which is significantly improved as compared to that of many previously reported fluorescence-based thrombin detection assays. Using other modified aptamers and antibodies instead of thrombin binding aptamers, this strategy may offer a suitable approach for detection of other proteins in biological, pharmaceutical and nano-mechanical applications.
Bailu Xu; Chuanqi Zhao; Weili Wei; Jinsong Ren; Daisuke Miyoshi; Naoki Sugimoto; Xiaogang Qu
Related Documents :
6220954 - Cardiac hypertrophy: biochemical and cellular relationships.
24096134 - N-acetyl cysteine blunts proteotoxicity in a heat shock protein-dependent manner.
24341994 - Lipids and topological rules governing membrane protein assembly.
25060114 - Amyloid transition of ubiquitin on silver nanoparticles produced by pulsed laser ablati...
20577724 - A novel dot-blot dnazyme-linked aptamer assay for protein detection.
3010904 - Interferon production in l929 cells under impaired translational conditions: comparison...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-10
Journal Detail:
Title:  The Analyst     Volume:  -     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization and Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, 130022, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Advances in optical biopsy - correlation of malignancy and cell density of primary brain tumors usin...
Next Document:  Core-shell magnetic nanoparticles: a comparative study based on silica and polydopamine coating for ...