Document Detail


Aptamer-based single-molecule imaging of insulin receptors in living cells.
MedLine Citation:
PMID:  24297059     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
ABSTRACT. We present a single-molecule imaging platform that quantitatively explores the spatiotemporal dynamics of individual insulin receptors in living cells. Modified DNA aptamers that specifically recognize insulin receptors (IRs) with a high affinity were selected through the SELEX process. Using quantum dot-labeled aptamers, we successfully imaged and analyzed the diffusive motions of individual IRs in the plasma membranes of a variety of cell lines (HIR, HEK293, HepG2). We further explored the cholesterol-dependent movement of IRs to address whether cholesterol depletion interferes with IRs and found that cholesterol depletion of the plasma membrane by methyl-β-cyclodextrin reduces the mobility of IRs. The aptamer-based single-molecule imaging of IRs will provide better understanding of insulin signal transduction through the dynamics study of IRs in the plasma membrane.
Authors:
Minhyeok Chang; Mijin Kwon; Sooran Kim; Na-Oh Yunn; Daehyung Kim; Sung Ho Ryu; Jong-Bong Lee
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  19     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2014 May 
Date Detail:
Created Date:  2013-12-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  51204     Citation Subset:  IM    
Affiliation:
Pohang University of Science & Technology, Department of Physics, Pohang 790-784, Republic of Korea.
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