Document Detail


Aptamer-based folding fluorescent sensor for cocaine.
MedLine Citation:
PMID:  11457319     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We adapted in two steps a deoxyribonucleotide-based aptamer to signal the recognition of cocaine: an instability was engineered in one stem of a three-way junction that forms the cocaine-binding pocket and the resulting short stem was end labeled with a fluorophore and a quencher. In the absence of cocaine, two stems are open, but in its presence they close and the three-way junction forms. This major structural change brings fluorophore and quencher together thereby signaling the presence and concentration of ligand. The sensor is selective for cocaine over its metabolites, can operate in serum, and is useful for the screening of cocaine hydrolases.
Authors:
M N Stojanovic; P de Prada; D W Landry
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  123     ISSN:  0002-7863     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-07-17     Completed Date:  2001-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4928-31     Citation Subset:  IM    
Affiliation:
Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, 630 West and 168th Street, New York, New York 10032, USA.
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MeSH Terms
Descriptor/Qualifier:
Cocaine / analysis*
Deoxyribonucleotides / chemistry*
Fluorescent Dyes / chemistry*
Chemical
Reg. No./Substance:
0/Deoxyribonucleotides; 0/Fluorescent Dyes; 50-36-2/Cocaine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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