Document Detail

Appraisal of Conjugated Linoleic Acid Production by Probiotic Potential of Pediococcus spp. GS4.
MedLine Citation:
PMID:  22971829     Owner:  NLM     Status:  Publisher    
Probiotics with ability to produce conjugated linoleic acid (CLA) is considered as an additive health benefit property for its known role in colon cancer mitigation. The conversion involves the biohydrogenation of the unsaturated fatty acid into conjugated form. Probiotic strain Pediococcus spp. GS4 was efficiently able to biohydrogenate linoleic acid (LA) into its conjugated form within 48 h of incubation. Quantum of CLA produced with a concentration of 121 μg/ml and sustained cell viability of 8.94 log cfu/ml maximally. Moreover, antibacterial effect of LA on the strain ability for biohydrogenation was examined at different concentrations and concluded to have a direct relationship between LA and amount of CLA produced. The efficiency of the strain for CLA production at different pH was also estimated and found maximum at pH 6.0 with 149 μg/ml while this ability was reduced at pH 9.0 to 63 μg/ml. Sesame oil, which is rich in the triacylglycerol form of LA, was also found to act as a substrate for CLA production by Pediococcus spp. GS4 with the aid of lipase-catalyzed triacylglycerol hydrolysis and amount of CLA produced was 31 μg/ml at 0.2 % while 150 μg/ml at 1.0 % of lipolysed oil in skim milk medium. Conjugated form was analyzed using UV scanning, RP-HPLC, and GC-MS. This study also focused on the alternative use of lipolysed sesame oil instead of costly LA for biohydrogenation and could be a potential source for the industrial production of CLA.
Vinay Dubey; Asit Ranjan Ghosh; Badal Kumar Mandal
Related Documents :
24261969 - Chirality sensing of amines, diamines, amino acids, amino alcohols, and α-hydroxy acid...
24242719 - Presence of a hydroxamic acid glucoside in wheat phloem sap, and its consequences for p...
17506569 - Formation of hydroxyphenyl-pyranoanthocyanins in grenache wines: precursor levels and e...
24421009 - Influence of carbohydrates and polyols on l-lactic acid production and fatty acid forma...
23411329 - Purification, properties and cdna cloning of glutamate decarboxylase in germinated faba...
10422769 - Effect of men 11467, a new tachykinin nk1 receptor antagonist, in acute rectocolitis in...
11960179 - Structure and anticoagulant properties of sulfated glycosaminoglycans from primitive ch...
15958719 - Multiple components of 2,4-dichlorophenoxyacetic acid uptake by rat choroid plexus.
23757329 - Towards a practical setup for hydrogen production from formic acid.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-13
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  -     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Center for Infectious Diseases and Control, School of Bio Sciences and Technology (SBST), VIT University, Vellore, 632014, India,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Altered regulatory mechanisms governing cell survival in children affected with clustering of autoim...
Next Document:  Effect of Acetic Acid on Saccharomyces Carlsbergensis ATCC 6269 Batch Ethanol Production Monitored b...