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Applications of microcalorimetry in the antibacterial activity evaluation of various Rhizoma coptidis.
MedLine Citation:
PMID:  21284509     Owner:  NLM     Status:  Publisher    
Context: Various Rhizoma coptidis are commonly used in clinical applications due to their strong antibacterial effect. Objective: The antibacterial effect of various R. coptidis were evaluated by microcalorimetry together with statistical analysis. Materials and methods: The dried roots of Coptis chinensis Franch, Coptis deltoidea C.Y. Cheng et Hsiao, and Coptis teeta Wall (Ranunculaceae) were identified by Prof. Xiao-He Xiao. The herbs were tested using thermal activity monitor (TAM) air isothermal microcalorimeter, ampoule mode, to evaluate the biological activity. The dynamic energy metabolism information about the Staphylococcus aureus growth at 37?C was analyzed with principal component analysis. Results: From the principle component analysis on quantitative parameters, the action of various R. coptidis on S. aureus growth at 37?C could be easily evaluated by analyzing the change of values of the two main parameters: the growth rate constant of first stage (k(1)) and the maximum heat rating of the first stage (P(1)). And the results of paired t-test show that there were no notable differences between inhibitory ratios calculated with k(1) and that of P(1). Using microdilution method, the minimal inhibitory concentrations (MIC) of C. chinensis, C. deltoidea, and C. teeta were detected, and they were 77.8???2, 116.6???4, 93.3???2 ?g/mL, respectively. Discussion and conclusion: This work provided a useful method for the evaluation of antibacterial activity of various R. coptidis.
Xue Feng; Dan Yan; Kui-Jun Zhao; Jiao-Yang Luo; Yong-Shen Ren; Wei-Jun Kong; Yu-Mei Han; Xiao-He Xiao
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-1
Journal Detail:
Title:  Pharmaceutical biology     Volume:  -     ISSN:  1744-5116     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9812552     Medline TA:  Pharm Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
China Military Institute of Chinese Materia Medica, Military Hospital of China, Beijing, People?s Republic of China.
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