Document Detail


Applications of automated amino acid analysis using 9-fluorenylmethyl chloroformate.
MedLine Citation:
PMID:  1687819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid and sensitive fully automated method for the determination of primary and secondary amino acids in different matrices is described. Amino acids are derivatized with 9-fluorenylmethyl chloroformate using an automated precolumn derivatization technique. Data are presented to show that the technique is both reproducible and highly sensitive. Applications of the technique are presented, including the analysis of peptide and protein hydrolysates and the profiling of free amino acids in physiological fluids.
Authors:
P A Haynes; D Sheumack; L G Greig; J Kibby; J W Redmond
Related Documents :
10606579 - Hplc method for analysis of free amino acids in fish using o-phthaldialdehyde precolumn...
17875379 - Development and validation of a liquid chromatographic method for the determination of ...
17089339 - Preparation and application of a new doubly tethered chiral stationary phase containing...
19808119 - Capillary electrochromatography with contactless conductivity detection for the determi...
11817729 - Sulfoacylated macro-porous polystyrene-divinylbenzene low-capacity cation exchanger sel...
4008569 - High-performance liquid chromatographic analysis of free hydroxyproline and proline in ...
7016849 - Saturated fatty acid-starved cells of saccharomyces cerevisiae grown in the presence of...
3342879 - 5-aminolevulinic acid formation from glutamate via the c5 pathway in clostridium thermo...
1035109 - Purification and some properties of a medium-chain acyl-thioester hydrolase from lactat...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  588     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1992-07-13     Completed Date:  1992-07-13     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  107-14     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Amino Acid Metabolism, Inborn Errors / blood
Amino Acids / analysis*,  blood
Autoanalysis / methods*
Child, Preschool
Chromatography, High Pressure Liquid / methods*
Drug Stability
Fluorenes*
Humans
Indicators and Reagents
Male
Microchemistry
Phenylketonurias / blood
Quality Control
Tyrosine / blood
Tyrosine Transaminase / deficiency
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Fluorenes; 0/Indicators and Reagents; 28920-43-6/1-(9-fluorenyl)methyl chloroformate; 42HK56048U/Tyrosine; EC 2.6.1.5/Tyrosine Transaminase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Simultaneous determination of centbutindole and its hydroxy metabolite in serum by high-performance ...
Next Document:  High-performance affinity chromatography of messenger RNA.