Document Detail

Applications of automated amino acid analysis using 9-fluorenylmethyl chloroformate.
MedLine Citation:
PMID:  1687819     Owner:  NLM     Status:  MEDLINE    
A rapid and sensitive fully automated method for the determination of primary and secondary amino acids in different matrices is described. Amino acids are derivatized with 9-fluorenylmethyl chloroformate using an automated precolumn derivatization technique. Data are presented to show that the technique is both reproducible and highly sensitive. Applications of the technique are presented, including the analysis of peptide and protein hydrolysates and the profiling of free amino acids in physiological fluids.
P A Haynes; D Sheumack; L G Greig; J Kibby; J W Redmond
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  588     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1992-07-13     Completed Date:  1992-07-13     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  107-14     Citation Subset:  IM    
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MeSH Terms
Amino Acid Metabolism, Inborn Errors / blood
Amino Acids / analysis*,  blood
Autoanalysis / methods*
Child, Preschool
Chromatography, High Pressure Liquid / methods*
Drug Stability
Indicators and Reagents
Phenylketonurias / blood
Quality Control
Tyrosine / blood
Tyrosine Transaminase / deficiency
Reg. No./Substance:
0/Amino Acids; 0/Fluorenes; 0/Indicators and Reagents; 28920-43-6/1-(9-fluorenyl)methyl chloroformate; 42HK56048U/Tyrosine; EC Transaminase

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