Document Detail

Application of a tri-enzyme extraction for total folate determination in foods.
MedLine Citation:
PMID:  2125600     Owner:  NLM     Status:  MEDLINE    
A tri-enzyme digestion procedure using chicken pancreas conjugase, alpha-amylase, and Pronase was evaluated to determine its usefulness in the microbiological quantitation of total folate in foods. Folate values obtained by traditional conjugase digestion were compared to those obtained by the tri-enzyme method for 12 food products that represent diverse matrixes. The tri-enzyme treatment increased measurable folate from most foods when compared to levels found after conjugase digestion. Largest increases were noted for tuna fish (51%) and yogurt (33%) after tri-enzyme digestion. For the 12 foods, a mean increase of 19% in measurable folate was obtained with tri-enzyme treatment. The study shows that traditional conjugase treatment does not completely free folate from complex food matrixes before microbiological analysis. Further, as other investigations have suggested, current accepted methods for folate analysis may be underestimating folate levels in foods.
J I Martin; W O Landen; A G Soliman; R R Eitenmiller
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal - Association of Official Analytical Chemists     Volume:  73     ISSN:  0004-5756     ISO Abbreviation:  J Assoc Off Anal Chem     Publication Date:    1990 Sep-Oct
Date Detail:
Created Date:  1991-02-28     Completed Date:  1991-02-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7505559     Medline TA:  J Assoc Off Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  805-8     Citation Subset:  IM    
Food and Drug Administration, Atlanta Center for Nutrient Analysis, GA 30309.
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MeSH Terms
Culture Media
Folic Acid / analysis*
Food Analysis*
Indicators and Reagents
Lactobacillus casei / metabolism
Nephelometry and Turbidimetry
Pronase / diagnostic use
Regression Analysis
alpha-Amylases / diagnostic use
gamma-Glutamyl Hydrolase / diagnostic use
Reg. No./Substance:
0/Culture Media; 0/Indicators and Reagents; 59-30-3/Folic Acid; EC; EC Hydrolase; EC 3.4.24.-/Pronase

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