| Application of a tri-enzyme extraction for total folate determination in foods. | |
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MedLine Citation:
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PMID: 2125600 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A tri-enzyme digestion procedure using chicken pancreas conjugase, alpha-amylase, and Pronase was evaluated to determine its usefulness in the microbiological quantitation of total folate in foods. Folate values obtained by traditional conjugase digestion were compared to those obtained by the tri-enzyme method for 12 food products that represent diverse matrixes. The tri-enzyme treatment increased measurable folate from most foods when compared to levels found after conjugase digestion. Largest increases were noted for tuna fish (51%) and yogurt (33%) after tri-enzyme digestion. For the 12 foods, a mean increase of 19% in measurable folate was obtained with tri-enzyme treatment. The study shows that traditional conjugase treatment does not completely free folate from complex food matrixes before microbiological analysis. Further, as other investigations have suggested, current accepted methods for folate analysis may be underestimating folate levels in foods. |
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Authors:
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J I Martin; W O Landen; A G Soliman; R R Eitenmiller |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal - Association of Official Analytical Chemists Volume: 73 ISSN: 0004-5756 ISO Abbreviation: J Assoc Off Anal Chem Publication Date: 1990 Sep-Oct |
Date Detail:
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Created Date: 1991-02-28 Completed Date: 1991-02-28 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7505559 Medline TA: J Assoc Off Anal Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 805-8 Citation Subset: IM |
Affiliation:
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Food and Drug Administration, Atlanta Center for Nutrient Analysis, GA 30309. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chickens Culture Media Folic Acid / analysis* Food Analysis* Hydrolysis Indicators and Reagents Lactobacillus casei / metabolism Nephelometry and Turbidimetry Pronase / diagnostic use Regression Analysis alpha-Amylases / diagnostic use gamma-Glutamyl Hydrolase / diagnostic use |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Indicators and Reagents; 59-30-3/Folic Acid; EC 3.2.1.1/alpha-Amylases; EC 3.4.19.9/gamma-Glutamyl Hydrolase; EC 3.4.24.-/Pronase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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