Document Detail

Application of tissue-engineered cartilage with BMP-7 gene to repair knee joint cartilage injury in rabbits.
MedLine Citation:
PMID:  19855958     Owner:  NLM     Status:  MEDLINE    
Injured articular cartilage has a poor capacity for spontaneous healing. So far, satisfactory solution to this subsistent problem has not been found, but transgenic therapy may be a promising way. This study aims to evaluate the effectiveness of a tissue-engineered cartilage that was transfected with morphogenetic protein 7 (BMP 7) in repairing the cartilaginous defects of rabbit knee joints. Chondrocytes were transfected with BMP-7 gene (5 x 10(6) cells/ml), inoculated into the collagen-fibrin gel scaffolds, and cultured for 14 days. Then, the scaffolds were implanted onto the created defects (5.0 mm in diameter) in rabbits' knee joints. After 12 weeks, the rabbits were sacrificed and histological sections were evaluated using modified O'Driscoll cartilage scores; In situ hybridization and immunohistochemistry were performed to detect the expression of BMP-7 mRNA and BMP-7 at the implanted site while the content of DNA and GAG was determined as well. A better quality of repairs was observed at the 12th week after implantation when compared to the control group using histological analyses. The content of DNA and specific secretion of GAG in the treatment group is statistically significant different compared with the control group. Gene therapy may be a promising treatment method, but the novel therapy approach needs further studies with respect to a longer follow-up period.
J H Che; Z R Zhang; G Z Li; W H Tan; X D Bai; F J Qu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-24
Journal Detail:
Title:  Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA     Volume:  18     ISSN:  1433-7347     ISO Abbreviation:  Knee Surg Sports Traumatol Arthrosc     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-16     Completed Date:  2010-12-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9314730     Medline TA:  Knee Surg Sports Traumatol Arthrosc     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  496-503     Citation Subset:  IM    
The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.
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MeSH Terms
Bone Morphogenetic Protein 7* / genetics,  therapeutic use
Cartilage, Articular / injuries*,  surgery*
Chondrocytes / transplantation*
Disease Models, Animal
Follow-Up Studies
Glycosaminoglycans / analysis
Knee Injuries / surgery*
Tissue Engineering / methods*
Tissue Scaffolds
Transfection / methods
Treatment Outcome
Reg. No./Substance:
0/BMP7 protein, human; 0/Bone Morphogenetic Protein 7; 0/Glycosaminoglycans

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