| Application of small interfering RNAs modified by unlocked nucleic acid (UNA) to inhibit the heart-pathogenic coxsackievirus B3. | |
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MedLine Citation:
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PMID: 20005874 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study describes the first application of unlocked nucleic acid (UNA)-modified small interfering RNAs (siRNAs) directed against a medically relevant target, the coxsackievirus B3. We systematically analyzed the impact of different siRNA modification patterns and observed good compatibility of the introduction of UNA with the maintenance of high antiviral activity. Additionally, the polarity of an siRNA was successfully reversed by modulating the relative stability of the termini with locked nucleic acid (LNA) and UNA as shown in a reporter assay. The potency of the reversed siRNA against the full-length target was, however, too low to inhibit the infectious virus. Altogether, combined modification of siRNAs with LNA und UNA provides a promising approach to alter and improve properties of an siRNA. |
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Authors:
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Denise Werk; Jesper Wengel; Suzy L Wengel; Hans-Peter Grunert; Heinz Zeichhardt; Jens Kurreck |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-13 |
Journal Detail:
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Title: FEBS letters Volume: 584 ISSN: 1873-3468 ISO Abbreviation: FEBS Lett. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-27 Completed Date: 2010-02-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 591-8 Citation Subset: IM |
Copyright Information:
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2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Institute for Virology, Campus Benjamin Franklin, Charit?-University Medicine, Berlin, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antiviral Agents / pharmacology* Cercopithecus aethiops Enterovirus B, Human / drug effects*, genetics Heart / virology* Nucleic Acids / chemistry* RNA, Small Interfering / chemistry*, genetics, pharmacology* Vero Cells |
| Chemical | |
Reg. No./Substance:
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0/Antiviral Agents; 0/Nucleic Acids; 0/RNA, Small Interfering |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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