| Application of a sensitive near-field microwave microprobe to the nondestructive characterization of microbial rhodopsin. | |
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MedLine Citation:
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PMID: 22517728 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We study the opto-electrical properties of Natronomonas pharaonis sensory rhodopsin II (NpSRII) by using a near-field microwave microprobe (NFMM) under external light illumination. To investigate the possibility of application of NFMM to biological macromolecules, we used time dependent properties of NPSRII before/after light activation which has three distinct states - ground-state, M-state, and O-state. The diagnostic ability of NFMM is demonstrated by measuring the microwave reflection coefficient (S(11) ) spectrum of NpSRII under steady-state illumination in the wavelength range of 350-650 nm. Moreover, we present microwave reflection coefficient S(11) spectra in the same wavelength range for two fast-photocycling rhodopsins: green light-absorbing proteorhodopsin (GPR) and Gloeobacter rhodopsin (GR). In addition the frequency sweep shift can be detected completely even for tiny amounts of sample (∼10(-3) OD of rhodopsin). Based on these results NFMM shows both very high sensitivity for detecting conformational changes and produces a good time-resolved spectrum. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim). |
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Authors:
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Songhui Kim; Youngwoon Yoon; Hanju Lee; Ah Reum Choi; Kwang-Hwan Jung; Arsen Babajanyan; Tigran Abrahamyan; Hyungkeun Yoo; Jung-Ha Lee; Deokjoon Cha; Gerard Berthiau; Barry Friedman; Kiejin Lee |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-20 |
Journal Detail:
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Title: Journal of biophotonics Volume: - ISSN: 1864-0648 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101318567 Medline TA: J Biophotonics Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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Department of Physics, Kunsan National University, Gunsan 573-701, Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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