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Application of a reversible immortalization system for the generation of proliferation-controlled cell lines.
MedLine Citation:
PMID:  19003263     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
To employ physiological mechanisms to control cell growth primary cells were reversibly immortalized using the SV40 TAg. The cells showed a fibroblast-like morphology. When the expression of the TAg was turned off, the cells arrested in the G0/G1 cell cycle phase. The cell culture could be kept for over 1 week in the proliferation-controlled state while the growth arrest remained fully reversible. The regulation was highly efficacious in that the arrested cell population did not spontaneously resume growth, suggesting that in the absence of the immortalizing gene expression endogenous growth-control mechanisms can keep these cells in a viable state for a prolonged time. Recombinant protein expression increased in growth-controlled cells when compared to conventionally cultured cells. Analysis of a secreted pharmaceutical protein revealed high product integrity without any signs of degradation. Therefore, it is feasible to apply genetic regulation of cell immortalization to obtain proliferation-controlled cell lines and this technique may be of interest to generate novel biotechnological producer cells.
Authors:
Tobias May; Werner Lindenmaier; Dagmar Wirth; Peter P Mueller
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Publication Detail:
Type:  Journal Article     Date:  2005-11-30
Journal Detail:
Title:  Cytotechnology     Volume:  46     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2008-11-12     Completed Date:  2011-07-14     Revised Date:  2013-03-12    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  69-78     Citation Subset:  -    
Affiliation:
Department of Gene Regulation and Differentiation, GBF - National, Research Center for Biotechnology, Mascheroder Weg 1, D-38124, Braunschweig, Germany.
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