| Application of recombinant phage display antibody system in study of Codakia orbicularis gill proteins. | |
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MedLine Citation:
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PMID: 15834161 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We used the recombinant phage display antibody system (RPAS) to obtain chimeric single-chain fragment variable (ScFv) antibodies to gill proteins of the white clam Codakia orbicularis (Linné, 1758). After three rounds of selection on immunotubes loaded with total gill protein extract, recombinant phages exhibiting antibodies to gill proteins were isolated and tested by enzyme-linked immunosorbent assay (ELISA). Clones exhibiting a high affinity for the mollusk proteins were selected for production of soluble ScFv antibodies, which were purified for subsequent analysis. ScFv antibodies exhibited a reaction specific for a protein whose molecular mass was about 15,000 Daltons and that was detected by the antigen capture technique followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blotting. |
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Authors:
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Jean-Philippe Gourdine; Pamela Greenwell; Emilie Smith-Ravin |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: 125 ISSN: 0273-2289 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-04-18 Completed Date: 2005-07-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 41-52 Citation Subset: IM |
Affiliation:
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Département de Biologie, Equipe DYNECAR EA 926, UFR Sciences Exactes et Naturelles, Université des Antilles et de la Guyane, 97 159 Pointe-à-Pitre, Guadeloupe. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Viral / immunology, isolation & purification Bacteriophages / immunology* Bivalvia / chemistry* Electrophoresis, Polyacrylamide Gel Genomic Library Gills / chemistry* Immunoassay / methods Immunoglobulin Variable Region / immunology Proteins / analysis* Recombination, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Viral; 0/Immunoglobulin Variable Region; 0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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