Document Detail

Application of recombinant fluorescent mammalian cells as a toxicity biosensor.
MedLine Citation:
PMID:  12227603     Owner:  NLM     Status:  MEDLINE    
With respect to developing a more sensitive biosensor, a recombinant fluorescent Chinese Hamster Ovary cell line was used for the monitoring of various toxicants. Both cell lines, EFC-500 and KFC-A10, were able to detect toxicants sensitively. They were characterized with mitomycin C and gamma-ray as genotoxicants and bisphenol A, nonylphenol, ziram and methyl bromide as possible and known EDCs. When compared to each other, the response of KFC-A10 was generally more informative and sensitive. Compared to typical bacterial biosensor systems, these cell lines offered a sensitivity of 2- to 50-fold greater for the tested chemicals. Based on these results, the use of mammalian cells offers a sensitive biosensor system that is not only fast, cheap and reproducible but also capable of monitoring the endocrine-like characteristics of environmental toxicants.
E J Kim; Y Lee; J E Lee; M B Gu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Water science and technology : a journal of the International Association on Water Pollution Research     Volume:  46     ISSN:  0273-1223     ISO Abbreviation:  Water Sci. Technol.     Publication Date:  2002  
Date Detail:
Created Date:  2002-09-13     Completed Date:  2003-01-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9879497     Medline TA:  Water Sci Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  51-6     Citation Subset:  IM    
Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology and the Advanced Environmental Monitoring Research Center, Puk-gu, Korea.
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MeSH Terms
Biological Assay / methods*
CHO Cells
Environmental Pollutants / toxicity*
Reproducibility of Results
Sensitivity and Specificity
Reg. No./Substance:
0/Environmental Pollutants

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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