| Application of a radioimmunoassay for determination of levels of zalcitabine (ddC) in human plasma, urine, and cerebrospinal fluid. | |
| | |
MedLine Citation:
|
PMID: 7695259 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A specific and sensitive radioimmunoassay for the determination of levels of zalcitabine in human plasma, urine, and cerebrospinal fluid has been developed. Commercially available radiolabel and antiserum (Sigma Chemicals) were used after dilution in assay buffer. Prior to the immunoassay, standard and patient samples were subjected to solid-phase extraction on silica columns in order to obtain purified samples. The lower limit of quantitation was determined to be 1 ng/ml. Intra- and interassay variations were less than 11% for a number of quality control samples of drug in plasma and urine. Results from parallelism studies with plasma and urine demonstrated that samples outside the standard range (1 to 30 ng/ml) could be diluted by blank plasma or assay buffer, respectively. A large number of related compounds and potentially coadministered drugs were tested for cross-reactivity. Stability tests showed that heat treatment for 30 min at 60 degrees C or storage for 1 month at -30 degrees C did not affect zalcitabine concentrations in plasma or urine. The radioimmunoassay with solid-phase extraction for sample cleanup discussed here has been successfully applied in a pharmacokinetic study of a single patient, demonstrating its applicability for clinical pharmacokinetic research with zalcitabine. |
| | |
Authors:
|
D M Burger; H Rosing; C H ten Napel; T Duyts; P L Meenhorst; J W Mulder; C H Koks; A Bult; J H Beijnen |
Related Documents
:
|
6238549 - Radioimmunoassay of nafarelin ([ 6-(3-(2-naphthyl)-d-alanine)]-luteinizing hormone-rele... 21047999 - Correlation between serum and plasma antibody titers to mycobacterial antigens. 7628089 - Assay of plasma testosterone during the first six months of life: importance of chromat... 17424829 - Development and validation of a sensitive radioimmunoassay procedure for estimating fsh... 1111169 - A competitive protein binding radioassay for deoxycorticosterone in human plasma. 18583619 - Plasma sphingosine-1-phosphate measurement in healthy subjects: close correlation with ... 8767729 - Studies and comparison of pterin patterns in the plasma of dogs and cats and their alte... 15255449 - Effect of age on plasma homocysteine concentrations in young and elderly subjects consi... 7732609 - Nonketotic hyperglycinemia in a newborn infant. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Antimicrobial agents and chemotherapy Volume: 38 ISSN: 0066-4804 ISO Abbreviation: Antimicrob. Agents Chemother. Publication Date: 1994 Dec |
Date Detail:
|
Created Date: 1995-04-27 Completed Date: 1995-04-27 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0315061 Medline TA: Antimicrob Agents Chemother Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 2763-7 Citation Subset: IM |
Affiliation:
|
Department of Pharmacy, Slotervaart Hospital, Amsterdam, The Netherlands. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Humans Middle Aged Radioimmunoassay Sensitivity and Specificity Zalcitabine / analysis*, pharmacokinetics |
| Chemical | |
Reg. No./Substance:
|
7481-89-2/Zalcitabine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pharmacokinetics of the triazole antifungal agent genaconazole in healthy men after oral and intrave...
Next Document: Quantification, qualification, and microbial killing efficiencies of antimicrobial chlorine-based su...