Document Detail

Application of platelet-rich plasma accelerates the wound healing process in acute and chronic ulcers through rapid migration and upregulation of cyclin A and CDK4 in HaCaT cells.
MedLine Citation:
PMID:  23242428     Owner:  NLM     Status:  In-Data-Review    
Application of autologous platelet-rich plasma (PRP) has been used for chronic wound healing. The aim of this study was to evaluate the effect of PRP on the wound healing processes of both acute and chronic ulcers and the underlying molecular mechanisms involved. We treated 16 patients affected by various acute and chronic ulcers with PRP. We performed molecular studies of cell proliferation, migration assays, immunoblotting and chloramphenicol acetyltransferase (CAT) assays in PRP-treated HaCaT keratinocyte cells. PRP treatment induced increased rates of cell proliferation and cell migration of HaCaT cells. In addition, the expression of cyclin A and cyclin dependent kinase (CDK) 4 proteins was markedly increased with a low concentration (0.5%) of PRP treatment in HaCaT cells. In 11 patients with chronic ulcers, including stasis ulcers, diabetic ulcers, venous leg ulcers, livedoid vasculitis, claw foot and traumatic ulcers, 9 patients showed 90-100% epithelization after 15.18 days. In 5 patients with acute ulcers, such as dehiscence, open wound and burn wound, 80-100% epithelization was achieved between 4 to 20 days. Topical application of PRP to acute and chronic skin ulcers significantly accelerated the epithelization process, likely through upregulation of the cell cycle regulatory proteins cyclin A and CDK4.
Sung-Ae Kim; Han-Won Ryu; Kyu-Suk Lee; Jae-We Cho
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Publication Detail:
Type:  Journal Article     Date:  2012-12-12
Journal Detail:
Title:  Molecular medicine reports     Volume:  7     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Report     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2012-12-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Report     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  476-80     Citation Subset:  IM    
Department of Dermatology, Keimyung University School of Medicine, Daegu 700-712, Republic of Korea.
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