| Application of molecular and serological assays to case based investigations of rubella and congenital rubella syndrome. | |
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MedLine Citation:
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PMID: 17516526 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rubella and congenital rubella syndrome continue to be important health problems worldwide. The detection of rubella RNA directly in clinical specimens is a critical factor in early laboratory diagnosis of recent or congenital infection, in addition to detection of rubella-specific IgM. In order to comply with recent WHO recommendations for establishing uniform genetic analysis protocols for rubella virus we have developed a new block based PCR assay (PCR-E317), which extends the sequence generated by the block based PCR-E592 currently in use, to cover the minimum acceptable 739 nucleotides (nt) window at the E1 gene. In addition, a real-time PCR assay has been developed to allow rapid detection of the virus in the laboratory. The assays were applied to a number of clinical specimens collected from patients including recent rubella incidences in the UK, Ethiopia and Turkey, two prenatal and two congenital rubella syndrome cases. Rubella RNA was detected in specimens from two patients that were collected too early for IgM detection, in two amniotic fluids for prenatal diagnosis and in the follow up specimens from the two infant with congenital rubella syndrome tested for viral secretion. At least four genotypes were identified among these patients. The results showed that molecular assays are important tools in the early diagnosis of rubella and congenital rubella syndrome, in the provision of molecular epidemiological information for tracking transmission pathways and in adding to the knowledge of rubella strain distribution worldwide. |
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Authors:
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Li Jin; Brenda Thomas |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of medical virology Volume: 79 ISSN: 0146-6615 ISO Abbreviation: J. Med. Virol. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-05-28 Completed Date: 2007-08-07 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7705876 Medline TA: J Med Virol Country: United States |
Other Details:
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Languages: eng Pagination: 1017-24 Citation Subset: IM |
Affiliation:
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Virus Reference Department, Centre for Infections, Health Protection Agency, London, UK. li.jin@hpa.org.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Amniotic Fluid / virology Antibodies, Viral / analysis Base Sequence Child Child, Preschool DNA Primers / genetics Female Genome, Viral Great Britain / epidemiology Humans Infant Infant, Newborn Middle Aged Molecular Epidemiology Phylogeny Polymerase Chain Reaction / methods, statistics & numerical data Pregnancy Prenatal Diagnosis / methods RNA, Viral / analysis, genetics Rubella / diagnosis*, epidemiology, immunology, virology Rubella Syndrome, Congenital / diagnosis*, epidemiology, immunology, virology Rubella virus / classification, genetics, immunology, isolation & purification Sensitivity and Specificity Serologic Tests |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Viral; 0/DNA Primers; 0/RNA, Viral; 0/rubella antibodies |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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