Document Detail


Application of liquid-liquid-liquid microextraction and ion-pair liquid chromatography coupled with photodiode array detection for the determination of chlorophenols in water.
MedLine Citation:
PMID:  18420216     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method termed as liquid-liquid-liquid microextraction was utilized to extract chlorophenols from water. The extracted chlorophenols, present in anionic form, were then separated, identified, and quantitated by ion-pair high-performance liquid chromatography with photodiode array detection (HPLC/DAD). For trace chlorophenol determination using HPLC/DAD, the chlorophenolate anion provides a better ultraviolet spectrum for quantitative and qualitative analyses than does uncharged chlorophenol. This is due to the auxochromic effect of the phenolate anion. In the study, experimental conditions such as organic phase identity, acceptor phase volume, sample agitation, extraction time, acceptor phase NaOH concentration, donor phase HCl concentration, salt addition, and UV absorption wavelength were optimized. Relative standard deviations (RSD, 2.3-5.4%), coefficients of determination (r2 0.9994-0.9999), and detection limits (0.049-0.081 ng mL(-1)) of the proposed method were investigated under the selected conditions. The method was successfully applied to analyses of reservoir and tap water samples, and the relative recoveries of chlorophenols from the spiked reservoir and tap water samples were 94.1-100.4% and 87.8-101.2%, respectively. The proposed method is capable of identifying and quantitating each analyte to 0.5 ng mL(-1), confirming the HPLC/DAD technique to be quite robust for monitoring trace levels of chlorophenols in water samples.
Authors:
Che-Yi Lin; Shang-Da Huang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-08
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1193     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-19     Completed Date:  2008-08-05     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  79-84     Citation Subset:  IM    
Affiliation:
Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Chlorophenols / analysis*
Chromatography, Liquid / methods*
Reproducibility of Results
Spectrophotometry, Ultraviolet
Water Pollutants, Chemical / analysis*
Chemical
Reg. No./Substance:
0/Chlorophenols; 0/Water Pollutants, Chemical

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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