Document Detail

Application of liquid-liquid-liquid microextraction and ion-pair liquid chromatography coupled with photodiode array detection for the determination of chlorophenols in water.
MedLine Citation:
PMID:  18420216     Owner:  NLM     Status:  MEDLINE    
A method termed as liquid-liquid-liquid microextraction was utilized to extract chlorophenols from water. The extracted chlorophenols, present in anionic form, were then separated, identified, and quantitated by ion-pair high-performance liquid chromatography with photodiode array detection (HPLC/DAD). For trace chlorophenol determination using HPLC/DAD, the chlorophenolate anion provides a better ultraviolet spectrum for quantitative and qualitative analyses than does uncharged chlorophenol. This is due to the auxochromic effect of the phenolate anion. In the study, experimental conditions such as organic phase identity, acceptor phase volume, sample agitation, extraction time, acceptor phase NaOH concentration, donor phase HCl concentration, salt addition, and UV absorption wavelength were optimized. Relative standard deviations (RSD, 2.3-5.4%), coefficients of determination (r2 0.9994-0.9999), and detection limits (0.049-0.081 ng mL(-1)) of the proposed method were investigated under the selected conditions. The method was successfully applied to analyses of reservoir and tap water samples, and the relative recoveries of chlorophenols from the spiked reservoir and tap water samples were 94.1-100.4% and 87.8-101.2%, respectively. The proposed method is capable of identifying and quantitating each analyte to 0.5 ng mL(-1), confirming the HPLC/DAD technique to be quite robust for monitoring trace levels of chlorophenols in water samples.
Che-Yi Lin; Shang-Da Huang
Related Documents :
18969626 - Study of the effect of different fiber coatings and extraction conditions on dry cured ...
24701366 - Determination of 16 selected trace elements in children plasma from china economical de...
12012176 - Miniaturized solid-phase extraction as a sample preparation technique for the determina...
20728896 - Optimisation of a simple and reliable method based on headspace solid-phase microextrac...
15811676 - Laboratory batch experiments of the combined effects of ultrasound and air stripping in...
446926 - Glucagon-like polypeptides in canine brain.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-08
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1193     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-19     Completed Date:  2008-08-05     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  79-84     Citation Subset:  IM    
Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chlorophenols / analysis*
Chromatography, Liquid / methods*
Reproducibility of Results
Spectrophotometry, Ultraviolet
Water Pollutants, Chemical / analysis*
Reg. No./Substance:
0/Chlorophenols; 0/Water Pollutants, Chemical

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Sol-gel methyl coating in capillary microextraction hyphenated on-line with high-performance liquid ...
Next Document:  Stable aqueous film coating dispersion of zein.