Document Detail


Application of ligand-exchange chromatography to the assay of L-alanine from DL-aspartic acid by Pseudomonas dacunhae.
MedLine Citation:
PMID:  1783629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A direct chiral ligand-exchange chromatographic method was developed to monitor L-alanine production by fermentation. A mobile phase containing aqueous 0.25 mM Zn2+ solution is utilized to separate amino acids in the fermentation medium. The detection limit for L-alanine is 0.5 ppm and the analysis time for one sample is about 8 min. As sample preparation is simple and the matrix effects are minimal, the assay is fast and convenient. The results indicate that the method has potential for the analysis of complicated fermentation media.
Authors:
C Y Cheng; L H Huang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  555     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1991 Aug 
Date Detail:
Created Date:  1992-03-16     Completed Date:  1992-03-16     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  272-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alanine / analysis*
Aspartic Acid / analysis*
Chromatography, Liquid / methods*
Copper
Pseudomonas / metabolism*
Zinc
Chemical
Reg. No./Substance:
30KYC7MIAI/Aspartic Acid; 789U1901C5/Copper; J41CSQ7QDS/Zinc; OF5P57N2ZX/Alanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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