Document Detail


Application of the immunoperoxidase technic to bone marrow trephine biopsies in the classification of patients with monoclonal gammopathies.
MedLine Citation:
PMID:  3085474     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study evaluated the utility of the immunoperoxidase method as applied to bone marrow sections in the diagnosis of patients with monoclonal gammopathies. Intracellular immunoglobulin light chains were identified in fixed, decalcified bone marrow biopsy sections from 66 patients with monoclonal proteins, using an avidin-biotin-peroxidase complex immunoperoxidase method. In all cases the predominant light chain identified in the bone marrow biopsy correlated with the monoclonal light chain identified in the serum. In addition, a light chain ratio was defined that correlated with the clinical diagnoses. The light chain ratios were highest in patients with multiple myeloma and were significantly different from those with monoclonal gammopathy of undetermined significance. There was no correlation between level of serum monoclonal protein and light chain ratios. The ratios were also high in patients with macroglobulinemia, primary amyloidosis, and renal disease secondary to monoclonal proteins but without overt myeloma. Determination of light chain ratios differentiated patients with multiple myeloma from those with monoclonal gammopathy of undetermined significance and helped identify patients with end organ damage secondary to monoclonal proteins but without overt myeloma.
Authors:
L C Peterson; B A Brown; J T Crosson; J Mladenovic
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  85     ISSN:  0002-9173     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  1986 Jun 
Date Detail:
Created Date:  1986-06-23     Completed Date:  1986-06-23     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  688-93     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Biopsy / methods*
Bone Marrow / pathology*
Humans
Hypergammaglobulinemia / classification*
Immunoenzyme Techniques*
Immunoglobulin Light Chains / analysis
Immunoglobulin kappa-Chains / analysis
Immunoglobulin lambda-Chains / analysis
Monoclonal Gammopathy of Undetermined Significance / classification*,  pathology
Multiple Myeloma / immunology
Waldenstrom Macroglobulinemia / immunology
Chemical
Reg. No./Substance:
0/Immunoglobulin Light Chains; 0/Immunoglobulin kappa-Chains; 0/Immunoglobulin lambda-Chains

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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