Document Detail

Application of hypothermia to autologous stem cell purging.
MedLine Citation:
PMID:  11578118     Owner:  NLM     Status:  MEDLINE    
Autologous stem cell transplantation is used widely after high-dose chemotherapy for treating hematological and other malignancies. Bone marrow harvested for autologous bone marrow transplantation may contain residual malignant cells even when the cancer is judged to be in remission. Attempts to purge marrow of its putative residual malignant cells may delay hemopoietic reconstitution and are of uncertain efficacy. In this report, we demonstrate the possibility of applying hypothermia to autologous stem cell purging. Using clonogenic assay, we compared the surviving fraction of human leukemia (HL60, K562) and human small cell lung cancer (H69) cell lines with that of normal human bone marrow CFU-GM and BFU-E cells after incubation at 4 +/- 0.1 degrees C for 24 and 48 h. Hypothermia decreased the surviving fraction of HL60, H69, and K562 cells. In contrast, the surviving fractions of stem cells were not affected by the temperature shift. The surviving fraction of HL60 cells at 4 degrees C cooling was significantly lower than that at 22 degrees C cooling. These findings suggest that in vitro hypothermia may selectively purge residual malignant cells in stored remission bone marrow and may be applicable before autologous bone marrow transplantation. In addition, the method is very simple and cost effective.
K Miyagi; T Yamazaki; I Tsujino; N Takahashi; Y Koya; M Masutani; U Sawada; T Horie
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryobiology     Volume:  42     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-10-01     Completed Date:  2001-12-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  190-5     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
The First Department of Internal Medicine, Nihon University School of Medicine, Tokyo, 173-8610, Japan.
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MeSH Terms
Bone Marrow Purging / methods*
Cold Temperature*
HL-60 Cells
Hematopoietic Stem Cell Transplantation*
K562 Cells
Transplantation, Autologous
Tumor Cells, Cultured
Tumor Stem Cell Assay

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