Document Detail


Application of hydrophobic anion-exchange phases in capillary electrochromatography.
MedLine Citation:
PMID:  11521866     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Capillary electrochromatography (CEC) requires stationary phases that enable appropriate electroosmotic propel under various conditions. Analyte retention can be controlled through hydrophobic or electrostatic interaction with the packing material. The development and characterization of new strong anion-exchange materials with additional hydrophobic moieties (SAX/C18 mixed-mode phases) is described. The synthesis was based on polymer encapsulation of porous silica. The phases were systematically characterized by means of elemental analyses, HPLC frontal analyses and CEC experiments. The studies focused on the influence of various parameters (e.g., pH, kind of buffer, capillary wall) on the electroosmotic flow (EOF). Phases with high anion-exchange capacity generated a fast and constant EOF over a wide pH range. Long-time stability of EOF and hydrophobic retention under CEC conditions were demonstrated within the course of 100 consecutive injections. The applicability of the SAX/C18 phases in appropriate buffer systems is demonstrated for neutral, acidic and basic compounds.
Authors:
B Scherer; F Steiner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  924     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-08-27     Completed Date:  2002-01-29     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  197-209     Citation Subset:  IM    
Affiliation:
University of the Saarland, Instrumental/Environmental Analysis, Saarbrücken, Germany.
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MeSH Terms
Descriptor/Qualifier:
Anion Exchange Resins*
Chromatography, Micellar Electrokinetic Capillary / instrumentation*
Chemical
Reg. No./Substance:
0/Anion Exchange Resins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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