Document Detail


Application of glutaraldehyde to in-cell Western assay for normalization.
MedLine Citation:
PMID:  20018162     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Normalization is essential to the in-cell Western (ICW) assay, a near-infrared immunocytoblot for protein analysis. Here we report that cells reacted with glutaraldehyde fluoresced in the near-infrared region of the spectrum, and the intensity of fluorescence was directly proportional to cell number over a range from 3125 to 100,000 cells per well. We took advantage of this property to develop a method for quantification of cells, and applied it to the ICW assay for normalization. The application of glutaraldehyde may make the ICW assay more popular due to the reduced cost and simplified procedure.
Authors:
Jingbao Li; Zhe Wang; Gang He; Wei Zhang; Airong Qian; Peng Shang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-14
Journal Detail:
Title:  Analytical biochemistry     Volume:  398     ISSN:  1096-0309     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-15     Completed Date:  2010-04-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  254-6     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2009 Elsevier Inc. All rights reserved.
Affiliation:
Key Laboratory for Space Bioscience and Biotechnology, Faculty of Life Sciences, Northwestern Polytechnical University, Xi'an, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Line, Tumor
Fibronectins / analysis,  immunology
Fluorescent Antibody Technique / methods,  standards*
Glutaral*
Humans
Mice
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/Fibronectins; 111-30-8/Glutaral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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