| Application of glutaraldehyde to in-cell Western assay for normalization. | |
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MedLine Citation:
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PMID: 20018162 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Normalization is essential to the in-cell Western (ICW) assay, a near-infrared immunocytoblot for protein analysis. Here we report that cells reacted with glutaraldehyde fluoresced in the near-infrared region of the spectrum, and the intensity of fluorescence was directly proportional to cell number over a range from 3125 to 100,000 cells per well. We took advantage of this property to develop a method for quantification of cells, and applied it to the ICW assay for normalization. The application of glutaraldehyde may make the ICW assay more popular due to the reduced cost and simplified procedure. |
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Authors:
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Jingbao Li; Zhe Wang; Gang He; Wei Zhang; Airong Qian; Peng Shang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-14 |
Journal Detail:
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Title: Analytical biochemistry Volume: 398 ISSN: 1096-0309 ISO Abbreviation: Anal. Biochem. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-15 Completed Date: 2010-04-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 254-6 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2009 Elsevier Inc. All rights reserved. |
Affiliation:
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Key Laboratory for Space Bioscience and Biotechnology, Faculty of Life Sciences, Northwestern Polytechnical University, Xi'an, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cell Line, Tumor Fibronectins / analysis, immunology Fluorescent Antibody Technique / methods, standards* Glutaral* Humans Mice Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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0/Fibronectins; 111-30-8/Glutaral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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