| Application of gelatin zymography for evaluating low levels of contaminating neutrophils in red blood cell samples. | |
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MedLine Citation:
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PMID: 20971053 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Supposedly "homogeneous" red blood cell (RBC) samples are commonly obtained by "washing" whole blood free of plasma, platelets, and white cells with physiological solutions, a procedure that does not result, however, in sufficient removal of polymorphonuclear neutrophils (PMNs), leading to possible artifactual results. Pure RBC samples can be obtained only by leukodepletion procedures. Proposed here is a version of gelatin zymography adapted to detect matrix metalloproteinase 9 (MMP-9), selectively expressed by PMNs, in heterogeneous mixtures of RBCs and PMNs that can reveal contamination at levels as low as 1 PMN/10⁶ RBCs. |
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Authors:
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Cesare Achilli; Annarita Ciana; Cesare Balduini; Angela Risso; Giampaolo Minetti |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-02 |
Journal Detail:
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Title: Analytical biochemistry Volume: 409 ISSN: 1096-0309 ISO Abbreviation: Anal. Biochem. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2010-12-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 296-7 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Biochemistry, University of Pavia, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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