Document Detail

Application of fluorescent techniques to evaluate the survival of probiotic lactobacilli to bile acid.
MedLine Citation:
PMID:  21365290     Owner:  NLM     Status:  MEDLINE    
To apply a fluorescent dye as an alternative technique to evaluate the survival of potentially probiotic lactobacilli to bile acids (BA) as first step in the design of probiotic functional foods. The use of lactic acid bacteria (LAB) in the functional food design depends on their ability to survive in the gastrointestinal tract where bile is an important natural barrier. Bile is mainly constituted by conjugated BA, which can be hydrolyzed to free BA and taurine or glycine. Changes in the transmembrane electrical potential (ΔΨ) of probiotic LAB strains due to the effect of conjugated and free BA were measured and showed that the majority of the tested LAB strains had greater sensibility to free BA than to their respective conjugated acids. Variations in the ΔΨ of the microorganism correlated well with bacterial viability determined by standard plate count method. We therefore propose the DiSC(3)-based fluorescent technique as a rapid and effective method to evaluate the resistance of probiotic lactobacilli to bile as first step for strain selection to be included in functional foods.
Ana Yanina Bustos; Raúl Raya; Elena Bru; Graciela Font de Valdez; María Pía Taranto
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-03-02
Journal Detail:
Title:  Biotechnology letters     Volume:  33     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-15     Completed Date:  2011-10-04     Revised Date:  2013-07-15    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1389-94     Citation Subset:  IM    
Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, San Miguel de Tucumán, Argentina.
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MeSH Terms
Anti-Bacterial Agents / toxicity*
Bacteriological Techniques / methods*
Bile Acids and Salts / toxicity*
Cell Membrane / drug effects
Fluorescent Dyes / metabolism
Lactobacillus / drug effects*
Membrane Potentials / drug effects
Microbial Viability / drug effects*
Staining and Labeling / methods*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bile Acids and Salts; 0/Fluorescent Dyes

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