Document Detail


Application of feedback-controlled bolus plus infusion (FC-B/I) method for quantitative PET imaging of dopamine transporters with [(18) F]β-CFT-FE in conscious monkey brain.
MedLine Citation:
PMID:  23042662     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The competitive inhibition of dopamine transporters (DAT) with cocaine, a specific DAT inhibitor, was evaluated with a feedback-controlled bolus plus infusion (FC-B/I) method using animal positron emission tomography (PET) in the living brain of conscious monkey. 2β-Carbomethoxy-3β-(4-fluorophenyl)-8-(2-[(18) F]fluoroethyl) nortropane ([(18) F]β-CFT-FE; Harada et al., 2004) was used for this study because it provided specific, fast and reversible kinetic properties to DAT in the striatum. In FC-B/I method, the real-time image reconstruction was started just after intravenous bolus injection of [(18) F]β-CFT-FE to generate a time-activity curve (TAC) in the striatum, and the infusion rate was adjusted to achieve an equilibrium state of the striatal radioactivity concentrations by means of a feedback-control algorithm. The first equilibrium state in the brain was reached within 20 min after the infusion start. Intravenous administration of cocaine at the doses of 0.02, 0.1 and 0.5 mg/kg shifted the equilibrium radioactivity level to the second equilibrium state in a dose-dependent manner, while no significant alterations was observed in the cerebellum. The present results demonstrated that the combined use of FC-B/I method and PET probe with fast kinetics like [(18) F]β-CFT-FE could be useful to assess the occupancy of drugs in the living brain with PET. Synapse, 2012. © 2012 Wiley Periodicals, Inc.
Authors:
Norihiro Harada; Hiroyuki Ohba; Takeharu Kakiuchi; Hideo Tsukada
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-8
Journal Detail:
Title:  Synapse (New York, N.Y.)     Volume:  -     ISSN:  1098-2396     ISO Abbreviation:  Synapse     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806914     Medline TA:  Synapse     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Central Research Laboratory, Hamamatsu Photonics K.K. 5000 Hirakuchi, Hamakita, Hamamatsu, Shizuoka 434-8601, JAPAN.
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