Document Detail


Application of factorial design to the study of xylitol production from eucalyptus hemicellulosic hydrolysate.
MedLine Citation:
PMID:  11456294     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study deals with the bioconversion of xylose into xylitol by Candida guilliermondii FTI 20037 using eucalyptus hemicellulosic hydrolysate obtained by acid hydrolysis. The influence of various parameters (ammonium sulfate, rice bran, pH, and xylose concentration) on the production of xylitol was evaluated. The experiments were based on multivariate statistical concepts, with the application of factorial design techniques to identify the most important variables in the process. The levels of these variables were quantified by the response surface methodology, which permitted the establishment of a significant mathematical model with a coefficient determination of R2 = 0.92. The best results (xylitol = 10.0 g/L, yield factor = 0.2 g/g, and productivity = 0.1 g/[L x h]) were attained with hydrolysate containing ammonium sulfate (1.1 g/L), rice bran (5.0 g/L), and xylose (initial concentration of 60.0 g/L), after 72 h of fermentation. The pH of fermentation was adjusted to 8.0 and the inoculum level utilized was 3 g/L.
Authors:
E V Canettieri; J B Almeida e Silva; M G Felipe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  94     ISSN:  0273-2289     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-07-17     Completed Date:  2001-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  159-68     Citation Subset:  IM    
Affiliation:
Department of Biotechnology, FAENQUIL, Lorena, SP, Brazil. ccanet@uol.com.br
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MeSH Terms
Descriptor/Qualifier:
Ammonium Sulfate / pharmacology
Candida / metabolism
Eucalyptus / chemistry*
Fermentation
Hydrogen-Ion Concentration
Models, Statistical
Multivariate Analysis
Polysaccharides / chemistry*
Protein Hydrolysates / chemistry
Xylitol / biosynthesis*,  chemistry*
Chemical
Reg. No./Substance:
0/Polysaccharides; 0/Protein Hydrolysates; 7783-20-2/Ammonium Sulfate; 8024-50-8/hemicellulose; 87-99-0/Xylitol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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