Document Detail


Application of external micro-spectrophotometric detection to improve sensitivity on microchips.
MedLine Citation:
PMID:  19649619     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The goal of this work was to increase the sensitivity of a UV-Vis spectrophotometer by decreasing the background noise and lengthening the optical path. A microphotometer has been modified to precisely select very small parts of a microfluidic channel pattern of a chip and to measure light absorbance on a magnified area of the selected part of the channel. The viability of combining a projection microscope and a spectrophotometer for external absorbance measurements on disposable PDMS chips was studied. Besides the external direct detection above a microfluidic channel, the optical pathlength was lengthened by detecting in the region of the perpendicular exit port. Increasing the cross-sectional area of the zone of irradiation improved the signal-to-noise ratio and the limits of detection (LOD).
Authors:
Attila Gáspár; István Bácsi; Erika F Garcia; Mihály Braun; Frank A Gomez
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Publication Detail:
Type:  Journal Article     Date:  2009-08-01
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  395     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-17     Completed Date:  2009-10-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  473-8     Citation Subset:  -    
Affiliation:
Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem tér 1, Debrecen, 4032, Hungary. gaspara@tigris.unideb.hu
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