Document Detail

Application of an enzyme immunoassay for the quantitative determination of azo dye (Orange II) in food products.
MedLine Citation:
PMID:  22889210     Owner:  NLM     Status:  Publisher    
This paper reports the preparation of polyclonal antibodies against a synthetic azo dye, Orange II, and the development of an indirect ELISA to detect Orange II in foods. The sulfonic group of Orange II was modified and linked with carrier protein to synthesise an artificial antigen. Based on the checkerboard titration, the method showed excellent sensitivity (IC(50) = 0.61  ng g(-1)) to Orange II in the linear range of 0.05-10 ng g(-1). The antibody had little cross-reactivity with Chromotrope FB, Gardenia Yellow, Ponceau 4R, Sunset Yellow and Sudan dyes. The ELISA had limits of detection (LOD) of 0.22, 0.97 and 0.74 ng g(-1) in chilli powder, chilli oil and braised pork, respectively. The limits of quantification (LOQ) of the assay were 0.91 ng g(-1) in chilli powder, 1.48 ng g(-1) in chilli oil and 1.10 ng g(-1) in braised pork. For food products fortified with 1-10 ng g(-1) Orange II, the inter- and intra-assay variations were all less than 24.0% and 18.0%, respectively. Therefore, the proposed test could be used as a rapid screening method for Orange II detection in food samples.
Huyin Xue; Yue Xing; Yongmei Yin; Taichang Zhang; Bo Zhang; Yu Zhang; Pei Song; Xi Tian; Yinghui Xu; Peng Wang; Meng Meng; Rimo Xi
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-13
Journal Detail:
Title:  Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment     Volume:  -     ISSN:  1944-0057     ISO Abbreviation:  Food Addit Contam Part A Chem Anal Control Expo Risk Assess     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101485040     Medline TA:  Food Addit Contam Part A Chem Anal Control Expo Risk Assess     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
a College of Pharmacy, State Key Laboratory of Medicinal Chemical Biology and Tianjin Key Laboratory of Molecular Drug Research, Nankai University , Tianjin , China.
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