| Application of dynamic diffractive optics for enhanced femtosecond laser based cell transfection. | |
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MedLine Citation:
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PMID: 20583035 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We demonstrate the advantages of a dynamic diffractive optical element, namely a spatial light modulator (SLM) for the controlled and enhanced optoinjection and phototransfection of mammalian cells with a femtosecond light source. The SLM provides full control over the lateral and axial positioning of the beam with sub-micron precision. Fast beam translation enables time-sequenced irradiation, which is shown to enhance the optoinjection efficiency and alleviate the problem of exact beam positioning on the cell membrane. We show that irradiation in three axial positions doubles the number of viably optoinjected cells when compared with a single dose. The presented system also enables untargeted raster scan irradiation which provides a higher throughput transfection of adherent cells at the rate of 1 cell per second. Additionally, fluorescent imaging is used to demonstrate cell selective two-step gene therapy. |
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Authors:
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Maciej Antkowiak; Maria Leilani Torres-Mapa; Frank Gunn-Moore; Kishan Dholakia |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biophotonics Volume: 3 ISSN: 1864-0648 ISO Abbreviation: J Biophotonics Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-22 Completed Date: 2011-01-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101318567 Medline TA: J Biophotonics Country: Germany |
Other Details:
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Languages: eng Pagination: 696-705 Citation Subset: IM |
Affiliation:
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SULSA, School of Biology, University of St Andrews, St Andrews KY169TS, UK. ma81@st-andrews.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals CHO Cells Cell Adhesion Cricetinae Cricetulus Lasers* Light* Optical Processes* Radiation Dosage Scattering, Radiation* Time Factors Transfection / instrumentation, methods* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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