Document Detail


Application of cytochrome b DNA sequences for the authentication of endangered snake species.
MedLine Citation:
PMID:  14687773     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to enforce the conservation program and curbing the illegal trading and consumption of endangered snake species, the value of cytochrome b sequence in the authentication of snake species was evaluated. As an illustration, DNA was extracted, selected cytochrome b DNA sequences amplified and sequenced from six snakes commonly consumed in Hong Kong. Cataloging with sequences available in public, a cytochrome b database containing 90 species of snakes was constructed. In this database, sequence homology between snakes ranged from 70.68 to 95.11%. On the other hand, intraspecific variation of three tested snakes was 0-0.98%. Using the database, we were able to determine the identity of six meat samples confiscated by the Agriculture, Fisheries and Conservation Department, HKSAR.
Authors:
Ka-Lok Wong; Jun Wang; Paul Pui-Hay But; Pang-Chui Shaw
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Forensic science international     Volume:  139     ISSN:  0379-0738     ISO Abbreviation:  Forensic Sci. Int.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2003-12-22     Completed Date:  2004-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7902034     Medline TA:  Forensic Sci Int     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  49-55     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Conservation of Natural Resources / legislation & jurisprudence*
Cytochromes b / genetics*
DNA, Mitochondrial / genetics*
Databases, Factual
Hong Kong
Internationality
Phylogeny
Snakes / classification,  genetics*
Species Specificity
Chemical
Reg. No./Substance:
0/DNA, Mitochondrial; 9035-37-4/Cytochromes b

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