| Application of a constant blood withdrawal method in equine exercise physiology studies. | |
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MedLine Citation:
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PMID: 11720024 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the present study was to test a constant blood withdrawal method (CBWM) to collect blood samples from horses during treadmill exercise. CBWM was performed in 4 Standardbreds and 5 Haflinger horses. A peristaltic pump was used to control blood aspiration from an i.v. catheter via an extension line. Blood was collected using an automatic fractions collector, with a constant delay time between the drawing of blood and sample collection. Blood withdrawal using CBWM was made during a treadmill standardised exercise test (SET). A blood flow of 12 m/min was used and samples collected every 60 s during the entire period of exercise. The volume of blood collected in each sample tube was 12.1+/-0.2 ml, with a delay time of mean +/- s.d. 25.3+/-0.8 s. Plasma lactate kinetics based on measurement of lactate in each fraction showed an exponential increase during the first 13 min of exercise (10.5 min of SET and 2.5 min recovery). The peak plasma lactate concentration was observed between 2.5 and 5.5 min after the end of SET. CBWM permits the kinetics of lactate and other blood-borne variables to be studied over time. This method could be a valuable aid for use in studying equine exercise physiology. |
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Authors:
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P Baragli; D Tedeschi; D Gatta; F Martelli; C Sighieri |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Equine veterinary journal Volume: 33 ISSN: 0425-1644 ISO Abbreviation: Equine Vet. J. Publication Date: 2001 Nov |
Date Detail:
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Created Date: 2001-11-26 Completed Date: 2002-04-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0173320 Medline TA: Equine Vet J Country: England |
Other Details:
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Languages: eng Pagination: 543-6 Citation Subset: IM |
Affiliation:
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Department of Anatomy, Biochemistry and Veterinary Physiology, University of Pisa, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Flow Velocity / veterinary Blood Specimen Collection / methods, veterinary* Catheterization / veterinary Exercise Test / veterinary Horses / blood*, physiology Jugular Veins Lactates / blood*, pharmacokinetics Male Physical Conditioning, Animal / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Lactates |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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