| Application of confocal laser scanning microscopy (CLSM) to visualize prolactin (PRL) and PRL mRNA in the normal and estrogen-treated rat pituitary glands using non-fluorescent probes. | |
| | |
MedLine Citation:
|
PMID: 9361267 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In the present study, we performed concomitant visualization of immunohistochemistry (IHC) and in situ hybridization (ISH) on the materials processed for conventional light microscopic specimens using non-fluorescent Confocal Laser. Scanning Microscopy (CLSM). CLSM was used in the reflection confocal mode using horseradish peroxidase (HRP)-3-3'diaminobenzidine (DAB)-osmium (osmium black) and nitroblue tetrazolium (NBT) as non-fluorescent detection methods (probes). To obtain clearer images of the organelles, images that were built up as electronic signals in CLSM were processed in an image analysis system (IAS). By using the combination of CLSM and IAS, in IHC, immunohistochemical localization of prolactin (PRL) was in well-developed lamellar or whorling rough endoplasmic reticula (RER), Golgi apparatus, and secretory granules. With ISH, the expression and distribution of PRL messenger ribonucleic acid (mRNA) was observed in a fashion suggesting polysome-like structures on RER. These observations were confirmed by immunoelectron microscopy and electron microscopic ISH. The herein-described method is expected to be useful to perform the concomitant observation of IHC and ISH at subcellular levels using the conventional light microscopic specimens. |
| | |
Authors:
|
J Itoh; N Sanno; A Matsuno; Y Itoh; K Watanabe; R Y Osamura |
Related Documents
:
|
6993687 - Improved handling of structural fragile cell-biological specimens during electron micro... 16933647 - The effects of nanomaterials on microstructures of sludge ash cement paste. 3305957 - Techniques for combining light microscopy and scanning electron microscopy: a survey of... 1338237 - Quantitative and computer assisted electron microscopic and microprobe studies in derma... 21519577 - Wear of ball attachments after 1 to 8 years of clinical use: a qualitative analysis. 15112547 - Original magnetic alignment of a nematic phase containing single-walled nanotubes. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Microscopy research and technique Volume: 39 ISSN: 1059-910X ISO Abbreviation: Microsc. Res. Tech. Publication Date: 1997 Oct |
Date Detail:
|
Created Date: 1997-12-17 Completed Date: 1997-12-17 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 9203012 Medline TA: Microsc Res Tech Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 157-67 Citation Subset: IM |
Affiliation:
|
Laboratories for Structure and Function Research, School of Medicine, Tokai University, Kanagawa, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cytoplasmic Granules / metabolism Endoplasmic Reticulum, Rough / metabolism Estrogens / pharmacology* Female Golgi Apparatus / metabolism Image Processing, Computer-Assisted Immunohistochemistry / methods In Situ Hybridization Lasers* Microscopy, Confocal / methods* Microscopy, Electron Microscopy, Immunoelectron Pituitary Gland / cytology, metabolism*, ultrastructure* Prolactin / analysis* RNA, Messenger / analysis* Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
|
0/Estrogens; 0/RNA, Messenger; 9002-62-4/Prolactin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Paracrine communication in the anterior pituitary as studied in reaggregate cell cultures.
Next Document: Transcription factors in normal and neoplastic pituitary tissues.