Document Detail

Application of cloned satellite DNA sequences to molecular-cytogenetic analysis of constitutive heterochromatin heteromorphisms in man.
MedLine Citation:
PMID:  3475246     Owner:  NLM     Status:  MEDLINE    
The cloned alpha-satellite DNA sequences were used to evaluate the specificity and possible variability of repetitive DNA in constitutive heterochromatin of human chromosomes. Five probes with high specificity to individual chromosomes (chromosomes 3, 11, 17, 18, and X) were in situ hybridized to metaphase chromosomes of different individuals. The stable position of alpha-satellite DNA sequences in heterochromatic regions of particular chromosomes was found. Therefore, the chromosome-specific alpha-satellite DNA sequences may be used as molecular markers for heterochromatic regions of certain human chromosomes. The homologous chromosomes of many individuals were characterized by cytologically visible heteromorphisms of hybridization intensity with chromosome-specific alpha-satellite DNA sequences. A special analysis of hybridization between homologues with morphological differences provided the evidence for a high resolution power of the in situ hybridization technique for evaluation of chromosome heteromorphisms. The approaches for detection of heteromorphisms in cases without morphological differences between homologues are discussed. The results obtained indicate that constitutive heterochromatin of human chromosomes has a variable amount of alpha-satellite DNA sequences. In situ hybridization of cloned satellite DNA sequences may be used as a new general approach to analysis of chromosome heteromorphisms in man.
Y B Yurov; S P Mitkevich; I A Alexandrov
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human genetics     Volume:  76     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-09-01     Completed Date:  1987-09-01     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  157-64     Citation Subset:  IM    
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MeSH Terms
Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 18
Chromosomes, Human, Pair 3
Cloning, Molecular*
DNA, Satellite / genetics*
Genetic Markers
Heterochromatin / genetics*
Nucleic Acid Hybridization
Polymorphism, Genetic*
X Chromosome
Reg. No./Substance:
0/DNA, Satellite; 0/Genetic Markers; 0/Heterochromatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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