Document Detail

Application of the chemiluminescent assay to cytotoxicity test: detection of menadione-catalyzed H2O2 production by viable cells.
MedLine Citation:
PMID:  1481979     Owner:  NLM     Status:  MEDLINE    
Menadione-catalyzed H2O2 production by viable cells is proportional to viable cell number. The correlations between the viable cell number and the concentration of H2O2 produced are determined with the rapid chemiluminescent assay (S. Yamashoji, T. Ikeda, and K. Yamashoji, 1989, Anal. Biochem. 181, 149-152). This chemiluminescent assay of viable cells requires only 10 min and is much faster than NR (neutral red) inclusion and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reduction assays, which require 3-5 h. When viable cells are incubated with antitumor drugs, detergents, mycotoxins, and glycoalkaloids for 24-48 h, a decrease in menadione-catalyzed H2O2 production in a dose- or incubation time-dependent manner is observed. In general, the 50% inhibition concentration determined by the chemiluminescent assay is lower than that determined by NR inclusion and MTT reduction assays, and the order of relative cytotoxic effects of agents is the same among these assays. Furthermore, clear cytotoxic effects are observed by the chemiluminescent assay after 1 h exposure of trypsinized cells to toxic compounds. Therefore, the chemiluminescent assay is expected to be more useful for the rapid detection of cytotoxic compounds than NR inclusion and MTT reduction assays.
S Yamashoji; F Nishimoto; M Usuda; H Kubota; K Isshiki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  207     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-02-11     Completed Date:  1993-02-11     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  255-60     Citation Subset:  IM    
Institute of King Brewing, Kakogawa, Japan.
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MeSH Terms
3T3 Cells
Antineoplastic Agents / pharmacology*
Cell Count / methods
Cell Line
Cell Survival / drug effects*
Chemiluminescent Measurements
Coloring Agents
Hydrogen Peroxide / metabolism*
Indicators and Reagents
Prostatic Neoplasms
Tetrazolium Salts
Tomatine / pharmacology
Tumor Cells, Cultured
Vitamin K / pharmacology*
Reg. No./Substance:
0/Antineoplastic Agents; 0/Coloring Agents; 0/Indicators and Reagents; 0/Tetrazolium Salts; 0/Thiazoles; 12001-79-5/Vitamin K; 17406-45-0/Tomatine; 298-93-1/thiazolyl blue; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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