Document Detail

Application of charge-coupled device technology for measurement of laser light and fluorescence distribution in tumors for photodynamic therapy.
MedLine Citation:
PMID:  1886937     Owner:  NLM     Status:  MEDLINE    
Laser and fluorescence light distributions with applications for photodynamic therapy were measured in mouse tumors using a non-invasive electronic optical imaging system. The system consists of a liquid-nitrogen-cooled, charge-coupled-device (CCD) array camera under computer control with 576 x 384 detection elements having dimensions of 23 microns x 23 microns. The available dynamic range of the array is approx. 10(3), and the effective wavelength range is 400-1000 nm. An interstitially placed cylindrical diffusing optical fiber was used to provide tumor illumination. The light distribution pattern from the fiber was determined by immersing the cylindrical diffusing tip in a fluorescing solution and recording the emission image. Fluorescence imaging facilitates an accurate measurement of light intensity distribution while avoiding problems associated with the directional nature of other detection methods used with diffusing fibers. Radiation-induced fibrosarcoma tumors on C3H mice were grown to about 1 cm diameter for in vivo recording of light distribution from the tumor volume and for determination of effective light penetration distance at 18 wavelengths in the range 458-995 nm. Endogenous tumor fluorescence and Photofrin II fluorescence intensity were measured over the wavelength range 585-725 nm to investigate the possible application of CCD imaging technology for drug distribution measurements. Model experiments were begun to evaluate the relative importance of potential distortions of light distribution measurements using this approach.
R C Straight; R E Benner; R W McClane; P M Go; G Yoon; J A Dixon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  53     ISSN:  0031-8655     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:  1991 Jun 
Date Detail:
Created Date:  1991-10-04     Completed Date:  1991-10-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  787-96     Citation Subset:  IM    
VAMC/University of Utah Laser Institute, Department of Medicine, Salt Lake City 84132.
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MeSH Terms
Fibrosarcoma / diagnosis,  drug therapy,  pathology*
Laser Therapy
Mice, Inbred C3H
Neoplasms, Radiation-Induced / diagnosis,  drug therapy,  pathology
Sarcoma, Experimental / diagnosis,  drug therapy,  pathology*
Spectrometry, Fluorescence / methods

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