| Application of carbon-ion beams or gamma-rays on primary tumors does not change the expression profiles of metastatic tumors in an in vivo murine model. | |
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MedLine Citation:
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PMID: 19362239 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To clarify how carbon-ion radiotherapy (C-ion) on primary tumors affects the characteristics of subsequently arising metastatic tumor cells. METHODS AND MATERIALS: Mouse squamous cell carcinomas, NR-S1, in synergic C3H/HeMsNrs mice were irradiated with a single dose of 5-50 Gy of C-ion (290 MeV per nucleon, 6-cm spread-out Bragg peak) or gamma-rays ((137)Cs source) as a reference beam. The volume of the primary tumors and the number of metastatic nodules in lung were studied, and histologic analysis and microarray analysis of laser-microdissected tumor cells were also performed. RESULTS: Including 5 Gy of C-ion and 8 Gy of gamma-rays, which did not inhibit the primary tumor growth, all doses used in this study inhibited lung metastasis significantly. Pathologic findings showed no difference among the metastatic tumor nodules in the nonirradiated, C-ion-irradiated, and gamma-ray-irradiated groups. Clustering analysis of expression profiles among metastatic tumors and primary tumors revealed a single cluster consisting of metastatic tumors different from their original primary tumors, indicating that the expression profiles of the metastatic tumor cells were not affected by the local application of C-ion or gamma-ray radiotherapy. CONCLUSION: We found no difference in the incidence and histology, and only small differences in expression profile, of distant metastasis between local C-ion and gamma-ray radiotherapy. The application of local radiotherapy per se or the type of radiotherapy applied did not influence the transcriptional changes caused by metastasis in tumor cells. |
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Authors:
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Tomoaki Tamaki; Mayumi Iwakawa; Tatsuya Ohno; Kaori Imadome; Miyako Nakawatari; Minako Sakai; Hirohiko Tsujii; Takashi Nakano; Takashi Imai |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: International journal of radiation oncology, biology, physics Volume: 74 ISSN: 1879-355X ISO Abbreviation: Int. J. Radiat. Oncol. Biol. Phys. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-13 Completed Date: 2009-04-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7603616 Medline TA: Int J Radiat Oncol Biol Phys Country: United States |
Other Details:
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Languages: eng Pagination: 210-8 Citation Subset: IM |
Affiliation:
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RadGenomics Research Group, Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, Chiba, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon Radioisotopes / therapeutic use* Carcinoma, Squamous Cell / genetics, pathology, radiotherapy*, secondary* Gamma Rays / therapeutic use* Lung Neoplasms / genetics, secondary* Male Mice Mice, Inbred C3H Neoplasms / genetics, pathology, radiotherapy* Oligonucleotide Array Sequence Analysis / methods RNA, Neoplasm / isolation & purification Radiotherapy Dosage Reverse Transcriptase Polymerase Chain Reaction / methods Time Factors Tumor Burden |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/RNA, Neoplasm |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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