| Application of capillary electrophoresis for organic acid analysis in herbal studies. | |
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MedLine Citation:
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PMID: 11504059 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A capillary electrophoresis (CE) procedure has been developed for the separation of 25 inorganic and organic acid anions using a buffer system consisting of 15 mM tris(hydroxymethyl)aminomethane, 3 mM 1,2,4-benzenetricarboxylic acid, 1.5 mM tetraethylenepentaamine (TEPA) and 20% methanol with pH adjusted to 8.4. A good separation of organic acids extracted from a mixture of Chinese traditional medicine (TCM) containing three herbs, Flos chrysthemi, Spica prunellae, and Folium mori was obtained using the procedure developed with satisfactory working range (0.20-77 mg/g), low detection limit (90-190 microg/g), and good repeatability (relative standard deviation 4.47-6.99%, n = 4). A satisfactory extraction of organic acids was achieved within 20 min using 0.1 M NaOH. The addition of TEPA to provide a reduced electroosmotic flow (EOF) environment was shown to remove interfering organic compounds extracted from TCM. The applicability of using organic acids as markers for determining the mixing ratio of constituent herbs for a TCM mixture was investigated using a three-component mixture with a 1:1:1 mixing ratio. A satisfactory mixing ratio of 1.04:1.09:0.98 was obtained using the methodology developed based on organic acids as markers. The application of our method for determining more complicated TCM mixtures has been discussed. |
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Authors:
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Y S Fung; H S Tung |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Electrophoresis Volume: 22 ISSN: 0173-0835 ISO Abbreviation: Electrophoresis Publication Date: 2001 Jul |
Date Detail:
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Created Date: 2001-08-15 Completed Date: 2002-01-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: Germany |
Other Details:
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Languages: eng Pagination: 2242-50 Citation Subset: IM |
Affiliation:
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Centre of Applied Spectroscopy and Analytical Sciences, Department of Chemistry, The University of Hong Kong, Hong Kong, SAR, China. ysfung@hkucc.hku.hk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Buffers Carboxylic Acids / analysis* Drugs, Chinese Herbal / chemistry* Electrophoresis, Capillary / methods* Medicine, Chinese Traditional Plants, Medicinal / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Buffers; 0/Carboxylic Acids; 0/Drugs, Chinese Herbal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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