Document Detail


Application of bleach method to improve sputum smear microscopy for the diagnosis of pulmonary tuberculosis.
MedLine Citation:
PMID:  16366115     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In an active surveillance study, the bleach concentration method to improve sputum smear microscopy for the diagnosis of pulmonary tuberculosis was applied in chest symptomatics in Mahidpur block of Ujjain district in Madhya Pradesh, India. We purposely selected twenty villages with population of approximately 20,000 individuals. 664 sputum specimens from 297 chest symptomatics were collected. Ziehl-Neelsen staining was performed on direct sputum smears, smears made after bleach method and modified Petroff's method. Out of 297 chest symptomatics, 16 cases (5.38%) were positive by direct microscopy, 27 cases (9.09%) were positive by bleach method, 22 cases (7.40%) were positive by modified Petroff's method. The bleach method is safe, cheap, easy and sensitive. It can be applied for improved detection of Mycobacterium tuberculosis in hospitals and laboratories especially in settings where mycobacterial culture facilities are not available. The implementation of bleach method clearly improves case detection and can be a useful contribution in the National Tuberculosis Control Program.
Authors:
Anubha Mutha; Samata Tiwari; Harish Khubnani; Sudha Mall
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article    
Journal Detail:
Title:  Indian journal of pathology & microbiology     Volume:  48     ISSN:  0377-4929     ISO Abbreviation:  Indian J Pathol Microbiol     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-12-21     Completed Date:  2006-01-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7605904     Medline TA:  Indian J Pathol Microbiol     Country:  India    
Other Details:
Languages:  eng     Pagination:  513-7     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Institute of Environmental Management and Plant Sciences, Vikram University, and R. D. Gardi Medical College, Surasa, Ujjain.
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MeSH Terms
Descriptor/Qualifier:
Bacteriological Techniques
Humans
India
Microscopy / methods
Mycobacterium tuberculosis / isolation & purification
Sodium Hypochlorite
Sputum / microbiology*
Tuberculosis, Pulmonary / diagnosis*,  microbiology
Chemical
Reg. No./Substance:
7681-52-9/Sodium Hypochlorite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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