| Application and analysis of structure-switching aptamers for small molecule quantification. | |
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MedLine Citation:
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PMID: 19327463 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Modern tools for the analysis of cellular function aim for the quantitative measurement of all members of a given class of biological molecules. Of the analyte classes, nucleic acid measurements are typically the most tractable, both on an individual analyte basis and in parallel. Thus, tools are being sought to enable measurement of other cellular molecules using nucleic acid biosensors. Of the variety of potential nucleic acid biosensor strategies, structure-switching aptamers (SSAs) present a unique opportunity to couple sensing and readout of the target molecule. However, little has been characterized about the parameters that determine the fidelity of the signal from SSA biosensors. In this study, a small molecule biosensor based on a SSA was engineered to detect the model small molecule, theophylline, in solution. Quantitative theophylline detection over nearly three orders-of-magnitude was achieved by scintillation counting and quantitative PCR. Further analysis showed that the biosensor fidelity is primarily controlled by the relative stability of the two conformations of the SSA. |
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Authors:
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Shengnan Xie; S Patrick Walton |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-02-21 |
Journal Detail:
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Title: Analytica chimica acta Volume: 638 ISSN: 1873-4324 ISO Abbreviation: Anal. Chim. Acta Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-03-30 Completed Date: 2009-06-23 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0370534 Medline TA: Anal Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 213-9 Citation Subset: IM |
Affiliation:
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Applied Biomolecular Engineering Laboratory, Department of Chemical Engineering and Material Science, Michigan State University, East Lansing, MI 48824, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aptamers, Nucleotide
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chemistry*,
genetics Biosensing Techniques / methods* Caffeine / analysis Nucleic Acid Conformation Polymerase Chain Reaction Reproducibility of Results Sensitivity and Specificity Theophylline / analysis* |
| Grant Support | |
ID/Acronym/Agency:
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CA126136/CA/NCI NIH HHS; GM079688/GM/NIGMS NIH HHS; R01 GM079688-03/GM/NIGMS NIH HHS; R21 CA126136-02/CA/NCI NIH HHS; R21 RR024439-01/RR/NCRR NIH HHS; RR024439/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aptamers, Nucleotide; 58-08-2/Caffeine; 58-55-9/Theophylline |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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