Document Detail


Application and analysis of the GFPu family of ubiquitin-proteasome system reporters.
MedLine Citation:
PMID:  16338377     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The relevance of the ubiquitin proteasome system (UPS) to disease and fundamental cellular processes has generated a demand for methods to monitor the activity of this system in living cells and organisms. Here we describe the GFP(u) family of UPS reporters. These reporters are constitutively degraded, ubiquitin-dependent proteasome substrates that can be used to monitor UPS function in the living cell. The GFP(u) reporter family consists of three variants that can report on global, nuclear, and cytoplasmic UPS function. This article focuses on the properties and design of these reporters and highlights appropriate techniques and applications for their use.
Authors:
Neil F Bence; Eric J Bennett; Ron R Kopito
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in enzymology     Volume:  399     ISSN:  0076-6879     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2005  
Date Detail:
Created Date:  2005-12-12     Completed Date:  2007-05-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  481-90     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Stanford University, Stanford, California, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Cell Line
Flow Cytometry
Genes, Reporter*
Green Fluorescent Proteins / chemistry,  genetics,  metabolism*
Humans
Molecular Sequence Data
Proteasome Endopeptidase Complex / metabolism*
Ubiquitin / metabolism*
Chemical
Reg. No./Substance:
0/Ubiquitin; 147336-22-9/Green Fluorescent Proteins; EC 3.4.25.1/Proteasome Endopeptidase Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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