| Application and analysis of the GFPu family of ubiquitin-proteasome system reporters. | |
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MedLine Citation:
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PMID: 16338377 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The relevance of the ubiquitin proteasome system (UPS) to disease and fundamental cellular processes has generated a demand for methods to monitor the activity of this system in living cells and organisms. Here we describe the GFP(u) family of UPS reporters. These reporters are constitutively degraded, ubiquitin-dependent proteasome substrates that can be used to monitor UPS function in the living cell. The GFP(u) reporter family consists of three variants that can report on global, nuclear, and cytoplasmic UPS function. This article focuses on the properties and design of these reporters and highlights appropriate techniques and applications for their use. |
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Authors:
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Neil F Bence; Eric J Bennett; Ron R Kopito |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in enzymology Volume: 399 ISSN: 0076-6879 ISO Abbreviation: Meth. Enzymol. Publication Date: 2005 |
Date Detail:
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Created Date: 2005-12-12 Completed Date: 2007-05-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0212271 Medline TA: Methods Enzymol Country: United States |
Other Details:
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Languages: eng Pagination: 481-90 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Stanford University, Stanford, California, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Cell Line Flow Cytometry Genes, Reporter* Green Fluorescent Proteins / chemistry, genetics, metabolism* Humans Molecular Sequence Data Proteasome Endopeptidase Complex / metabolism* Ubiquitin / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Ubiquitin; 147336-22-9/Green Fluorescent Proteins; EC 3.4.25.1/Proteasome Endopeptidase Complex |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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