| Application of acetate derivatives for gas chromatography-mass spectrometry: novel approaches on carbohydrates, lipids and amino acids analysis. | |
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MedLine Citation:
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PMID: 18783777 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The structure of glycoconjugates has been determined by several chromatographic methods, however gas chromatography-mass spectrometry (GC-MS) has been widely used to identify and quantify the volatile trimethylsilyl and fluoroacyl derivatives. Adapting the reduction/acetylation strategies, we had performed the derivatization of all monosaccharide class, as well as amino acids and OH-fatty acids as from different glycoconjugates. Uronic acids gave characteristic ions at m/z 143, 156 and 173, and 19 amino acids derivatives, gave molecular ions [M]+ and daughter ions of [M-59]+ and [M-43]+ on electron impact (EI)-MS, which provide their rapid identification. |
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Authors:
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Guilherme L Sassaki; Lauro M Souza; Rodrigo V Serrato; Thales R Cipriani; Philip A J Gorin; Marcello Iacomini |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-08-28 |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1208 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-20 Completed Date: 2008-12-09 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 215-22 Citation Subset: IM |
Affiliation:
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Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, Curitiba, Paraná, CEP 81531-980, Brazil. sassaki@ufpr.br |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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analysis* Amino Acids / analysis* Carbohydrates / analysis* Gas Chromatography-Mass Spectrometry / methods* Lipids / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Amino Acids; 0/Carbohydrates; 0/Lipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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