Document Detail

Application of X-ray micrography and imaging to study the effect of gentamicin on Pseudomonas aeruginosa.
MedLine Citation:
PMID:  9630409     Owner:  NLM     Status:  MEDLINE    
Aminoglycosides disrupt the outer membrane of Pseudomonas aeruginosa to facilitate access to their intracellular target. High-resolution X-ray micrography of live specimens is a relatively new technique. We used laser (nanosecond) plasma to image live cells of P. aeruginosa ATCC 27853. After exposure to 25 mg/L gentamicin for 15 min. we observed perturbation of the cell surface, membrane blebbings (370 nm and 273 nm diameter) away from the cell, formation of distinct channels (241 nm long) resulting from indentation and induction of cell elongation from 3-3.6 microm (control) to 4.6-5.26 microm (gentamicin-treated cells). These data illustrate the potential of high-resolution X-ray micrography for studying effects of drugs on live microbiological specimens.
J M Rajyaguru; D S Torres; E Abel; M C Richardson; M J Muszynski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of antimicrobial chemotherapy     Volume:  41     ISSN:  0305-7453     ISO Abbreviation:  J. Antimicrob. Chemother.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-07-29     Completed Date:  1998-07-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7513617     Medline TA:  J Antimicrob Chemother     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  557-61     Citation Subset:  IM    
Department of Pediatric Infectious Diseases Research, Nemours Childrens Clinic-Orlando, FL 32806, USA.
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MeSH Terms
Anti-Bacterial Agents / pharmacology*
Gentamicins / pharmacology*
Microscopy, Atomic Force
Pseudomonas aeruginosa / drug effects*,  ultrastructure
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Gentamicins

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