| Application of Western blotting for the detection of uncoupling protein-2 (UCP-2) in mitochondria from smokers and non-smokers | |
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MedLine Citation:
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PMID: 19462344 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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INTRODUCTION: Uncoupling proteins (UCPs) are a family of transmembrane anion transporters present in the inner mitochondrial membrane. UCP-2, which exhibits the widest distribution in various tissues, plays an important role in many physiological processes. Human UCP-2 studies have been hampered by the lack of a method for measuring this protein in an easily accessible human tissue, e.g. blood. The aim of this study was to develop such a method and test its utility by comparing UCP-2 levels in smokers and non-smokers. MATERIAL AND METHODS: Venous blood samples from 10 smoking and seven non-smoking volunteers were used for the study; lymphocytes were isolated employing Lymphoprep. UCP-2 levels were measured by Western blotting combined with chemoluminescence detection. RESULTS: Total lymphocyte homogenates were found useless for measuring UCP-2 levels, but it was possible to measure UCP-2 in homogenates of purified lymphocyte mitochondria. There was a significant, though moderate, linear correlation between UCP-2 level and daily cigarette use. UCP-2 level in peripheral blood lymphocytes from smokers was higher than that in non-smokers. CONCLUSION: The method for measuring UCP-2 in peripheral blood lymphocytes opens the possibility of UCP-2 screening studies in humans and thus may be useful for studying the role of the protein in human physiology and pathology. |
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Authors:
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Kinga I Stańczak-Mrozek; Monika Kolanowska; Paweł Grieb |
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Publication Detail:
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Type: Comparative Study; English Abstract; Journal Article |
Journal Detail:
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Title: Pneumonologia i alergologia polska : organ Polskiego Towarzystwa Ftyzjopneumonologicznego, Polskiego Towarzystwa Alergologicznego, i Instytutu Gruźlicy i Chorób Płuc Volume: 77 ISSN: 0867-7077 ISO Abbreviation: Pneumonol Alergol Pol Publication Date: 2009 |
Date Detail:
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Created Date: 2009-05-22 Completed Date: 2009-09-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9302892 Medline TA: Pneumonol Alergol Pol Country: Poland |
Other Details:
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Languages: pol Pagination: 118-22 Citation Subset: IM |
Affiliation:
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Zakład Farmakologii Doświadczalnej Instytutu Medycyny Doświadczalnej i Klinicznej PAN w Warszawie. kingastanczak@wp.pl |
Vernacular Title:
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Zastosowanie metody Western blot do oznaczania białka rozprzegajacego 2 (UCP-2) w mitochondriach osób palacych i niepalacych. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Blotting, Western / methods Female Humans Ion Channels / blood* Male Middle Aged Mitochondria / metabolism* Mitochondrial Proteins / blood* Multivariate Analysis Regression Analysis Smoking / blood* Tobacco Use Disorder / blood* Uncoupling Agents / blood* |
| Chemical | |
Reg. No./Substance:
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0/Ion Channels; 0/Mitochondrial Proteins; 0/Uncoupling Agents; 0/mitochondrial uncoupling protein 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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