| [Application of TRUST, TP-ELISA and TPPA in the serologic diagnosis of syphilis]. | |
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MedLine Citation:
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PMID: 23214254 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the application value of toluidine red unheated serum test (TRUST), treponema pallidum enzyme-linked immunosorbent assay (TP-ELISA) and treponema pallidum particle agglutination assay (TPPA) in the serologic diagnosis of syphilis. METHODS: We collected serum samples from 12 622 inpatients, 2 253 outpatients and 1 500 subjects in the medical examination center, and screened them by TRUST and TP-ELISA, followed by quantitative tests using TRUST and TPPA. RESULTS: Among the 16 375 cases, clinically diagnosed syphilis was confirmed in 384, with a positive rate of 100% either by TRUST (1:2 -1 :16) or TPPA (1:80 - 1:2 560), and 92.2% by TP-ELISA. CONCLUSION: TP-ELISA and TRUST should be simultaneously used for syphilis screening, but not TP-ELISA alone, and the results should be confirmed by TPPA. The combination of the three methods plays a valuable role in reducing the misdiagnosis and missed diagnosis, and contributes to the early and accurate diagnosis of syphilis. |
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Authors:
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Ting-Ting Wang; Qin Li; Jing-Feng Yu; Chen-Yue Xiao; Li-Ping Zhang |
Publication Detail:
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Type: English Abstract; Journal Article |
Journal Detail:
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Title: Zhonghua nan ke xue = National journal of andrology Volume: 18 ISSN: 1009-3591 ISO Abbreviation: Zhonghua Nan Ke Xue Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-12-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101093592 Medline TA: Zhonghua Nan Ke Xue Country: China |
Other Details:
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Languages: chi Pagination: 1020-2 Citation Subset: IM |
Affiliation:
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Department of Clinical Laboratory, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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