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Application of a Simple In-House PCR-SSP Technique for HLA-B* 27 Typing in Spondyloarthritis Patients.
MedLine Citation:
PMID:  24490069     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Background. Microlymphocytotoxicity (MLCT) and flowcytometry (FC) are the conventional serological methods to detect HLA-B* 27. Due to some disadvantages in these methods, most of the HLA laboratories have now switched over to molecular methods. Molecular techniques based on commercial kits are expensive; as such many laboratories with limited funds in developing countries cannot afford these techniques. Aims. Our main aim was to standardize a simple inexpensive in-house PCR-SSP technique for HLA-B* 27 typing. Materials and Methods. Sequence Specific primers were designed to amplify all the subtypes of B* 27 using IMGT-HLA sequence database. Accuracy was checked by retyping of 90 PCR-SSOP typed controls. Results. The presence of 149 bp specific band with control band on 2% agarose gel showed B* 27 positivity. No discrepancies were found when compared with PCR-SSOP results. The frequency of HLA-B* 27 was found to be significantly increased (68.75% versus 4.40%, O.R 46.909: P value 6.62E - 32) among 700 SpA patients as compared to controls. Clinically, 54% of patients had polyarticular arthritis with SI joints involvement (68%) and restricted spine flexion (60%). Conclusion. In-house PCR-SSP technique is very simple and inexpensive technique to detect B* 27 allele, which was strongly associated with SpA patients from Western India.
Authors:
Devraj J Parasannanavar; Anjali Rajadhyaksha; Kanjaksha Ghosh
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Publication Detail:
Type:  Journal Article     Date:  2013-12-19
Journal Detail:
Title:  Arthritis     Volume:  2013     ISSN:  2090-1984     ISO Abbreviation:  Arthritis     Publication Date:  2013  
Date Detail:
Created Date:  2014-02-03     Completed Date:  2014-02-03     Revised Date:  2014-02-05    
Medline Journal Info:
Nlm Unique ID:  101566207     Medline TA:  Arthritis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  504109     Citation Subset:  -    
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