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Application of SRAP markers in the identification of Stylosanthes guianensis hybrids.
MedLine Citation:
PMID:  24965147     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Sequence-related amplified polymorphism (SRAP) is a new molecular marker technology developed based on polymerase chain reaction. The authenticity of 84 progenies of 8 hybrid combinations of Stylosanthes guianensis was identified by SRAP markers to select the true hybrids used in the present study. A total of 35 SRAP primer combinations were selected from the parents of 8 hybrid combinations. The selected polymorphism primer combinations were applied to identify the authenticity of all progenies. The male parents of the primer combinations had specific markers, whereas the female parents did not. 68 progenies exhibited male parent-specific bands, which were identified as true hybrids. The rest of the progenies were considered self-hybrids because of the absence of male parent-specific bands. The results of hybrid identification provided solid evidence for further studies of hybrids and demonstrated SRAP molecular markers as a useful technology for assessing the purity of S. guianensis hybrids.
Authors:
C Q Huang; G D Liu; C J Bai; W Q Wang; J Tang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-6-26
Journal Detail:
Title:  Molecular biology reports     Volume:  -     ISSN:  1573-4978     ISO Abbreviation:  Mol. Biol. Rep.     Publication Date:  2014 Jun 
Date Detail:
Created Date:  2014-6-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0403234     Medline TA:  Mol Biol Rep     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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