Document Detail


Application of Raman spectroscopy for visualizing biochemical changes during peripheral nerve injury in vitro and in vivo.
MedLine Citation:
PMID:  24281358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Raman spectroscopy can be used for analysis of objects by detecting the vibrational spectrum using label-free methods. This imaging method was applied to analysis of peripheral nerve regeneration by examining the sciatic nerve in vitro and in vivo. Raman spectra of intact nerve tissue had three particularly important peaks in the range 2800-3000 cm-1. Spectra of injured sciatic nerves showed significant changes in the ratio of these peaks. Analysis of cellular spectra suggested that the spectrum for sciatic nerve tissue reflects the axon and myelin components of this tissue. Immunohistochemical analysis showed that the number of axons and the myelinated area were reduced at 7 days after injury and then increased by 28 days. The relative change in the axon to myelin ratio showed a similar initial increase, followed by a decrease at 28 days after injury. These changes correlated with the band intensity ratio and the changes in distribution of axon and myelin in Raman spectral analysis. Thus, our results suggest that label-free biochemical imaging with Raman spectroscopy can be used to detect turnover of axon and myelin in peripheral nerve regeneration.
Authors:
Shinsuke Morisaki; Chikashi Ota; Ken-ichi Matsuda; Natsuko Kaku; Hiroyoshi Fujiwara; Ryo Oda; Hidenobu Ishibashi; Toshikazu Kubo; Mitsuhiro Kawata
Related Documents :
17006688 - Impaired scaling of responses to vestibular stimulation in incomplete sci.
9928838 - Neck muscle activity after unilateral labyrinthectomy in the alert guinea pig.
8971498 - Relationship between reaction time of eye movement and activity of the neck extensors.
19135388 - Active pauses induce more variable electromyographic pattern of the trapezius muscle ac...
20083068 - Electroanatomic relationships in patients with primum atrioventricular septal defect.
19997868 - Development of a scaffoldless three-dimensional engineered nerve using a nerve-fibrobla...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  18     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-11-27     Completed Date:  2014-06-09     Revised Date:  2014-07-07    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  116011     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Axons / metabolism
Cells, Cultured
Ganglia, Spinal / chemistry,  cytology
Myelin Sheath / metabolism
Peripheral Nerve Injuries / metabolism*
Rats
Rats, Sprague-Dawley
Schwann Cells / chemistry,  cytology
Sciatic Nerve / chemistry*,  injuries*,  metabolism
Spectrum Analysis, Raman / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Stationary distribution of self-organized states and biological information generation.
Next Document:  Ocular and visual status among children in special schools in Wales: the burden of unrecognised visu...