Document Detail

Application of the RB-FPG technique for distinguishing early and late replicating X chromosomes in farm animals.
MedLine Citation:
PMID:  9670475     Owner:  NLM     Status:  MEDLINE    
The sex chromosomes of Murrah buffalo and Sahiwal cattle (females) were studied for R-banding patterns. A short term whole blood culture method was used for obtaining metaphase chromosomes. The R-banding method using 5-bromo-2'-deoxyuridine (BrdU), the fluorescent 33258 Hoechst stain followed by blue-black light exposure and Giemsa staining (RB-FPG) was applied to the chromosome preparations. The two X chromosomes in all the females were clearly distinguished. One of the chromosomes (early replicating) had alternative light and dark bands and the other X chromosome (late replicating) was either completely pale banded or had one or a few dark bands. The reason for this phenomenon could be BrdU incorporation in the late replicating X chromosome, which on exposure to blue light in the presence of 33258 Hoechst is destroyed and does not take the stain. Thus, one of the two X chromosomes shows differential staining behaviour.
J S Yadav; S Vats; B R Yadav
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytobios     Volume:  91     ISSN:  0011-4529     ISO Abbreviation:  Cytobios     Publication Date:  1997  
Date Detail:
Created Date:  1998-07-31     Completed Date:  1998-07-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0207227     Medline TA:  Cytobios     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  201-5     Citation Subset:  IM    
Department of Zoology, Kurukshetra University, Haryana, India.
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MeSH Terms
Animals, Domestic
Azure Stains
Chromosome Banding / methods*
DNA Replication*
Fluorescent Dyes
X Chromosome*
Reg. No./Substance:
0/Antimetabolites; 0/Azure Stains; 0/Fluorescent Dyes; 23491-44-3/Bisbenzimidazole; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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